https://scholar.korea.ac.kr/handle/2021.sw.korea/734 2026-04-05T15:37:30Z 2026-04-05T15:37:30Z Choi, Inyoung Choi, Hyelin Lee, Jung-Soo Han, Jaejoon https://scholar.korea.ac.kr/handle/2021.sw.korea/146445 2024-11-15T07:24:46Z 2023-03-15T00:00:00Z

Title: Novel color stability and colorimetry-enhanced intelligent CO2 indicators by metal complexation of anthocyanins for monitoring chicken freshness Authors: Choi, Inyoung; Choi, Hyelin; Lee, Jung-Soo; Han, Jaejoon Abstract: This study aims to improve the color stability of anthocyanins and develop a CO2-sensitive indicator based on black goji anthocyanin (BGA) extract. Although the BGA extracts showed distinct color changes, such as red-purple-blue, their intrinsic color diminished after 24 h. A metal complexation method was used for the high color stability of BGA. BGA extracts were chelated with various concentrations of Al3+ [0 - 20% (w/w)]. It showed high color stability and strong intensity in a dose-dependent manner. A CO2-sensitive indicator sachet was developed using hydroxypropyl methylcellulose hydrogel, based on 5% (w/w) Al3+-BGA complexes. The indicator was applied to the chicken breast and detected its spoilage after 3 days with its changing color to greyish blue, due to the microbial growth to 7.00 log CFU/g. These results demonstrated the possibility of chelated anthocyanin complexes as indicating dyes and the ability to monitor the food quality changes through noticeable color changes.

2023-03-15T00:00:00Z Min, Nayoung Park, Hahyun Hong, Taeyeon An, Garam Song, Gwonhwa Lim, Whasun https://scholar.korea.ac.kr/handle/2021.sw.korea/146448 2024-11-15T06:37:43Z 2023-02-05T00:00:00Z

Title: Developmental toxicity of prometryn induces mitochondrial dysfunction, oxidative stress, and failure of organogenesis in zebrafish (Danio rerio) Authors: Min, Nayoung; Park, Hahyun; Hong, Taeyeon; An, Garam; Song, Gwonhwa; Lim, Whasun Abstract: Prometryn, 2-methylthio-4,6-bis(isopropylamino)-1,3,5-triazine, is a selective thiomethyl triazine herbicide widely used to control unwanted weeds and harmful insects by inhibiting electron transport in target organisms. Despite having various advantages, herbicides pose as a major threat to the environment and human health due to persistent contamination, bioaccumulation, and damage to non-target organisms. In this study, the develop-mental toxicity of 5, 10, and 20 mg/L prometryn in zebrafish (Danio rerio) embryos was evaluated and compared to that of the solvent control for 96 h. Several transgenic zebrafish models (fli1a:eGFP, flk1:eGFP, olig2:dsRed and L-fabp:dsRed) were visually assessed to detect fluorescently tagged genes. Results showed that prometryn shortened body length, and induced yolk sac, heart edema, abnormal heart rate, and loss of viability. Fluores-cence microscopy revealed that prometryn exposure caused defects in organ development, reactive oxygen species accumulation, and apoptotic cell death. Mitochondrial bioenergetics were also evaluated to determine the effect of prometryn on the electron transport chain activity and metabolic alterations. Prometryn was foundto interfere with mitochondrial function, ultimately inhibiting energy metabolism and embryonic development. Collectively, our findings suggest that prometryn is a potential contaminate for non-target sites and organisms, especially aquatic, and emphasize the need to consider the toxic effects of prometryn.

2023-02-05T00:00:00Z Hong, Taeyeon Park, Hahyun An, Garam Song, Gwonhwa Lim, Whasun https://scholar.korea.ac.kr/handle/2021.sw.korea/146461 2024-11-15T17:20:48Z 2023-01-01T00:00:00Z

Title: Ethalfluralin induces developmental toxicity in zebrafish via oxidative stress and inflammation Authors: Hong, Taeyeon; Park, Hahyun; An, Garam; Song, Gwonhwa; Lim, Whasun Abstract: Ethalfluralin, of dinitroaniline herbicide family, is an effective weed controller. Following residue detection in herbicide-treated fields, ethalfluralin was reported to interfere with early stages of implantation in some vertebrate species. However, the role of ethalfluralin in the development of zebrafish embryos has not been elucidated yet. Therefore, in the present study, we investigated the morphological and physiological changes that occur in the embryonic development of zebrafish due to ethalfluralin exposure. Results indicated that ethalfluralin decreased survival rate along with reduction in the hatching ratio and heartbeat. It was observed to cause edema in the heart and yolk sac, and apoptosis in the anterior region of the developing zebrafish larvae; as visualized through acridine orange and TUNEL staining. In addition, ethalfluralin increased the expression of the apoptosis-associated genes including tp53, cyc1, casp8, casp9, and casp3. The Seahorse Mito Stress analysis revealed that ethalfluralin slightly reduced mitochondrial respiration in live zebrafish embryos. Reactive oxygen species (ROS) production was also observed to be elevated in zebrafish larvae in response to ethalfluralin. Treatment with ethalfluralin decreased blood vessel formation in brain and intestine in flk1 transgenic zebrafish embryos. The decrease in angiogenesis related gene expression was specifically observed in vegfc, flt1, and kdrl, and in the intestinal vasculature related genes apoa4a, aqp3, fabp2, and vil1. Moreover, an increase in infiammatory genes such as cox2a, cox2b, cxcl-c1c, il8, mcl1a, mcl1b, and nf-kappa b was observed using real-time PCR analysis. Collectively, these results indicate that oxidative stress generated by exposure to ethalfiuralin induced ROS generation, apoptosis, infiammation and anti-angiogenic effects, and therefore, ethalfiuralin may be toxic to the development of zebrafish embryos.

2023-01-01T00:00:00Z Shrestha, Prasansah Karmacharya, Jayram Han, So-Ra Park, Hyun Oh, Tae-Jin https://scholar.korea.ac.kr/handle/2021.sw.korea/143707 2024-11-15T06:15:42Z 2022-12-31T00:00:00Z

Title: In silico analysis and a comparative genomics approach to predict pathogenic trehalase genes in the complete genome of Antarctica Shigella sp. PAMC28760 Authors: Shrestha, Prasansah; Karmacharya, Jayram; Han, So-Ra; Park, Hyun; Oh, Tae-Jin Abstract: Although four Shigella species (S. flexneri, S. sonnei, S. dysenteriae, and S. boydii) have been reported, S. sp. PAMC 28760, an Antarctica isolate, is the only one with a complete genome deposited in NCBI database as an uncharacterized isolate. Because it is the world's driest, windiest, and coldest continent, Antarctica provides an unfavourable environment for microorganisms. Computational analysis of genomic sequences of four Shigella species and our uncategorized Antarctica isolates Shigella sp. PAMC28760 was performed using MP3 (offline version) program to predict trehalase encoding genes as a pathogenic or non-pathogenic form. Additionally, we employed RAST and Prokka (offline version) annotation programs to determine locations of periplasmic (treA) and cytoplasmic (treF) trehalase genes in studied genomes. Our results showed that only 56 out of 134 Shigella strains had two different trehalase genes (treF and treA). It was revealed that the treF gene tends to be prevalent in Shigella species. In addition, both treA and treF genes were present in our strain S. sp. PAMC28760. The main objective of this study was to predict the prevalence of two different trehalase genes (treF and treA) in the complete genome of Shigella sp. PAMC28760 and other complete genomes of Shigella species. Till date, it is the first study to show that two types of trehalase genes are involved in Shigella species, which could offer insight on how the bacteria use accessible carbohydrate like glucose produced from the trehalose degradation pathway, and importance of periplasmic trehalase involvement in bacterial virulence.

2022-12-31T00:00:00Z