https://scholar.korea.ac.kr/handle/2021.sw.korea/2442 2026-04-09T11:37:22Z https://scholar.korea.ac.kr/handle/2021.sw.korea/269319 Title: Bioinstructive scaffolds enhance stem cell engraftment for functional tissue regeneration Authors: Wu, Di; Eugenis, Ioannis; Hu, Caroline; Kim, Soochi; Kanugovi, Abhijnya; Yue, Shouzheng; Wheeler, Joshua R.; Fathali, Iman; Feeley, Sonali; Shrager, Joseph B.; Huang, Ngan F.; Rando, Thomas A. Abstract: Stem cell therapy is a promising approach for tissue regeneration after traumatic injury, yet current applications are limited by inadequate control over the fate of stem cells after transplantation. Here we introduce a bioconstruct engineered for the staged release of growth factors, tailored to direct different phases of muscle regeneration. The bioconstruct is composed of a decellularized extracellular matrix containing polymeric nanocapsules sequentially releasing basic fibroblast growth factor and insulin-like growth factor 1, which promote the proliferation and differentiation of muscle stem cells, respectively. When applied to a volumetric muscle loss defect in an animal model, the bioconstruct enhances myofibre formation, angiogenesis, innervation and functional restoration. Further, it promotes functional muscle formation with human or aged murine muscle stem cells, highlighting the translational potential of this bioconstruct. Overall, these results highlight the potential of bioconstructs with orchestrated growth factor release for stem cell therapies in traumatic injury. 2025-04-17T00:00:00Z https://scholar.korea.ac.kr/handle/2021.sw.korea/264859 Title: Protocol for quantifying muscle fiber size, number, and central nucleation of mouse skeletal muscle cross-sections using Myotally software Authors: Both, Pieter; Kim, Soochi; Kang, Jengmin; Arjona, Marina; Benjamin, Daniel I.; Nutter, Christopher W.; Goshayeshi, Armon; Rando, Thomas A. Abstract: Here, we present a protocol for using Myotally, a user-friendly software for fast, automated quantification of muscle fiber size, number, and central nucleation from immunofluorescent stains of mouse skeletal muscle cross-sections. We describe steps for installing the software, preparing compatible images, finding the file path, and selecting key parameters like image quality and size limits. We also detail optional features, such as measuring mean fluorescence. By automating these traditionally labor-intensive processes, Myotally improves research efficiency and data consistency. © 2024 The Author(s) 2025-03-21T00:00:00Z https://scholar.korea.ac.kr/handle/2021.sw.korea/127002 Title: The NCgl0127 Gene of Corynebacterium glutamicum Plays Role in Cysteine Metabolism Authors: Lee, Heung-Shick 2021-06-24T00:00:00Z https://scholar.korea.ac.kr/handle/2021.sw.korea/137812 Title: Comprehensive genomic analysis reveals virulence factors and antibiotic resistance genes in Pantoea agglomerans KM1, a potential opportunistic pathogen Authors: Guevarra, Robin B.; Magez, Stefan; Peeters, Eveline; Chung, Mi Sook; Kim, Kyung Hyun; Radwanska, Magdalena Abstract: Pantoea agglomerans is a Gram-negative facultative anaerobic bacillus causing a wide range of opportunistic infections in humans including septicemia, pneumonia, septic arthritis, wound infections and meningitis. To date, the determinants of virulence, antibiotic resistance, metabolic features conferring survival and host-associated pathogenic potential of this bacterium remain largely underexplored. In this study, we sequenced and assembled the whole-genome of P. agglomerans KM1 isolated from kimchi in South Korea. The genome contained one circular chromosome of 4,039,945 bp, 3 mega plasmids, and 2 prophages. The phage-derived genes encoded integrase, lysozyme and terminase. Six CRISPR loci were identified within the bacterial chromosome. Further in-depth analysis showed that the genome contained 13 antibiotic resistance genes conferring resistance to clinically important antibiotics such as penicillin G, bacitracin, rifampicin, vancomycin, and fosfomycin. Genes involved in adaptations to environmental stress were also identified which included factors providing resistance to osmotic lysis, oxidative stress, as well as heat and cold shock. The genomic analysis of virulence factors led to identification of a type VI secretion system, hemolysin, filamentous hemagglutinin, and genes involved in iron uptake and sequestration. Finally, the data provided here show that, the KM1 isolate exerted strong immunostimulatory properties on RAW 264.7 macrophages in vitro. Stimulated cells produced Nitric Oxide (NO) and pro-inflammatory cytokines TNF-alpha, IL-6 and the anti-inflammatory cytokine IL-10. The upstream signaling for production of TNF-alpha, IL-6, IL-10, and NO depended on TLR4 and TLR1/2. While production of TNF-alpha, IL-6 and NO involved solely activation of the NF-kappa B, IL-10 secretion was largely dependent on NF-kappa B and to a lesser extent on MAPK Kinases. Taken together, the analysis of the whole-genome and immunostimulatory properties provided in-depth characterization of the P. agglomerans KM1 isolate shedding a new light on determinants of virulence that drive its interactions with the environment, other microorganisms and eukaryotic hosts 2021-01-06T00:00:00Z