https://scholar.korea.ac.kr/handle/2021.sw.korea/2486 Sun, 05 Apr 2026 17:47:39 GMT 2026-04-05T17:47:39Z https://scholar.korea.ac.kr/handle/2021.sw.korea/266466 Title: Unveiling the biological activities of the microbial long chain hydroxy fatty acids as dual agonists of GPR40 and GPR120 Authors: Park, Yeeun; Woo, Ji-Min; Shin, Jaeeun; Chung, Myunghae; Seo, Eun-Ji; Lee, Sung-Joon; Park, Jin-Byung Abstract: The physiological functions of various fatty acid-originating metabolites from foods and fermented products remained mostly untouched. Thereby, this study examined the biological activities of hydroxy fatty acids as agonists of G protein-coupled receptors (i.e., GPR40 and GPR120), which are derived from long-chain fatty acids (e.g., oleic acid and linoleic acid) by microbiota. Cell-based Ca2+ mobilization assays and in silico docking simulations revealed that not only the degree of unsaturation but also the number and position of hydroxyl groups played a key role in their agonist activities. For instance, 8,11-dihydroxyoctadec-9Z-enoic acid exhibited significantly greater Ca2+ response in the GPR40/GPR120-expressing cells as compared to the endogenous agonists (e.g., linoleic acid and docosahexaenoic acid), forming hydrogen bond interactions with residues in the ligand-binding pockets of receptors. This study will contribute to understanding the relationships between fatty acid structures and agonist activities. Sat, 15 Feb 2025 00:00:00 GMT https://scholar.korea.ac.kr/handle/2021.sw.korea/266466 2025-02-15T00:00:00Z https://scholar.korea.ac.kr/handle/2021.sw.korea/267967 Title: Simultaneous Quantitation and Stability Evaluation of Cholecalciferol and α-Tocopherol in Vitamin-Fortified Nanoemulsions by UHPLC-MS/MS Coupled with QuEChERS Extraction Authors: Kim, Tae Eun; Lee, Jae Hoon; Kim, Yun Jeong; Lee, Min Hyeock; Jang, Hae Won; Chun, Yong Gi; Kim, Bum-Keun Abstract: A novel QuEChERS-UHPLC-MS/MS method was developed for the simultaneous quantification of cholecalciferol and α-tocopherol in vitamin-fortified nanoemulsions. Critical extraction parameters, including solvent volume, extraction time, agitation type, and QuEChERS salt composition, were optimized using recovery tests and a Box-Behnken design. The method demonstrated excellent linearity (R2 > 0.9999), low detection limits, high repeatability (0.12-1.96% RSD), reproducibility (0.58-1.97% RSD), and accuracy (97.18-106.21%), with negligible matrix effects (97.66-105.67%). The thermal stability of both vitamins was evaluated under different temperature treatments: low-temperature long-time (65 °C for 30 min), high-temperature short-time (95 °C for 15 s), and autoclave treatment (121 °C for 15 min). Significant degradation was observed only after autoclave treatment, while higher soy lecithin content in the nanoemulsions enhanced vitamin retention under heat stress. This method offers a reliable tool for the routine analysis and stability evaluation of vitamin-fortified nanoemulsions, contributing to their quality control and development. © 2025 American Chemical Society. Fri, 17 Jan 2025 00:00:00 GMT https://scholar.korea.ac.kr/handle/2021.sw.korea/267967 2025-01-17T00:00:00Z https://scholar.korea.ac.kr/handle/2021.sw.korea/200849 Title: Optimization of the QuEChERS-UPLC-APCI-MS/MS method for the analysis of vitamins D and K nanoencapsulated in yogurt Authors: Kim, Tae-Eun; Lee, Min Hyeock; Kim, Bum-Keun; Lee, Jae Hoon; Chun, Yong Gi; Jang, Hae Won Abstract: A novel approach was developed to simultaneously determine the contents of vitamins D-2, D-3, K-1, and K-2 in yogurt fortified with nanoencapsulated vitamins D and K. This method combines QuEChERS extraction with UPLC-APCI-MS/MS analysis. Optimization of the QuEChERS process included fine-tuning the addition of salts using response surface methodology based on the Box-Behnken design. Under the optimized conditions, the developed method exhibited an excellent linearity (R-2 > 0.999) across concentrations ranging from 0.5 to 500 mu g/L. The limits of detection and quantification (LOD and LOQ) were found to be 0.01-0.04 mu g/L and 0.04-0.11 mu g/L, respectively, with precision, accuracy, and recovery rates exceeding 94.88 %, and accompanied by acceptable relative standard deviations. Comparative analysis with traditional methodologies revealed the significant advantages of the proposed approach. Previous techniques such as liquid-liquid extraction combined with saponification are time-consuming and require high sample quantities. In addition, dispersive liquid-liquid microextraction requires a long analysis time and exhibits a poor sensitivity, particularly in terms of its LOD and LOQ values. In contrast, our method offers a straightforward, efficient, and reliable sample preparation technique suitable for detecting vitamins D-2, D-3, K-1, and K-2 in a yogurt matrix. This study not only demonstrates the feasibility of applying the QuEChERS method for stable vitamin quantification in yogurt, but it also represents an innovative contribution to enhancing the detection sensitivity and efficiency in food analysis. By emphasizing these methodological advancements and comparative benefits, this research underscores the significance of adopting advanced analytical approaches in food science. Fri, 11 Oct 2024 00:00:00 GMT https://scholar.korea.ac.kr/handle/2021.sw.korea/200849 2024-10-11T00:00:00Z https://scholar.korea.ac.kr/handle/2021.sw.korea/200978 Title: Nonanoic acid and cholecystokinin induce beige adipogenesis Authors: Park, Hyun Ji; Kim, Ji-Sun; Kim, Eun Ryung; Gu, Man Bock; Lee, Sung-Joon Abstract: Beige adipocytes, crucial for thermogenesis, offer a potential therapeutic strategy for obesity. This study investigated the anti-obesity effects of nonanoic acid (NoA), medium-chain fatty acids, and cholecystokinin-8 (CCK-8) on beige adipogenesis in C3H10T1/2 mesenchymal stem cells (C3H10T1/2 MSCs). We observed a significant increase in cholecystokinin B receptor expression in beige adipocytes compared to preadipocytes. The co-treatment with NoA and CCK-8 enhanced beige adipocyte differentiation and lipid accumulation. Moreover, the co-treatment with NoA and CCK-8 upregulated the mRNA expression of thermogenic genes and increased mitochondrial activity more effectively than individual treatment. Specifically, NoA and CCK-8 co-treatment also elevated the protein expression of uncoupling protein 1 and peroxisome proliferator-activated receptor-gamma coactivator-1 alpha. These findings suggest that the additive effect of NoA and CCK-8 promotes the beiging/browning of body fat in beige adipogenesis, potentially serving as an effective approach in the prevention and treatment of obesity and insulin resistance. Tue, 10 Sep 2024 00:00:00 GMT https://scholar.korea.ac.kr/handle/2021.sw.korea/200978 2024-09-10T00:00:00Z