https://scholar.korea.ac.kr/handle/2021.sw.korea/824 Fri, 10 Apr 2026 12:28:29 GMT 2026-04-10T12:28:29Z https://scholar.korea.ac.kr/handle/2021.sw.korea/143605 Title: Enhancing adoptive T-cell therapy with fucoidan-based IL-2 delivery microcapsules Authors: Jeon, Eun Young; Choi, Da-som; Choi, Seunghyun; Won, Ju-young; Jo, Yunju; Kim, Hye-bin; Jung, Youngmee; Shin, Sang Chul; Min, Hophil; Choi, Hae Woong; Lee, Myeong Sup; Park, Yoon; Chung, Justin J.; Jin, Hyung-seung Abstract: Adoptive cell therapy (ACT) with antigen-specific T cells is a promising treatment approach for solid cancers. Interleukin-2 (IL-2) has been utilized in boosting the efficacy of ACT. However, the clinical applications of IL-2 in combination with ACT is greatly limited by short exposure and high toxicities. Herein, a complex coacervate was designed to intratumorally deliver IL-2 in a sustained manner and protect against proteolysis. The complex coacervate consisted of fucoidan, a specific IL-2 binding glycosaminoglycan, and poly-l-lysine, a cationic counterpart (FPC2). IL-2-laden FPC2 exhibited a preferential bioactivity in ex vivo expansion of CD8(+)T cells over Treg cells. Additionally, FPC2 was embedded in pH modulating injectable gel (FPC2-IG) to endure the acidic tumor microenvironment. A single intratumoral administration of FPC2-IG-IL-2 increased expansion of tumor-infiltrating cytotoxic lymphocytes and reduced frequencies of myeloid populations. Notably, the activation and persistency of tumor-reactive T cells were observed only in the tumor site, not in the spleen, confirming a localized effect of FPC2-IG-IL-2. The immune-favorable tumor microenvironment induced by FPC2-IG-IL-2 enabled adoptively transferred TCR-engineered T cells to effectively eradicate tumors. FPC2-IG delivery system is a promising strategy for T-cell-based immunotherapies. Sun, 01 Jan 2023 00:00:00 GMT https://scholar.korea.ac.kr/handle/2021.sw.korea/143605 2023-01-01T00:00:00Z https://scholar.korea.ac.kr/handle/2021.sw.korea/146526 Title: Spatial template-based geometric complexity reduction method for photo-realistic modeling of large-scale indoor spaces Authors: Hyeon, Janghun; Kim, Joohyung; Choi, Hyunga; Jang, Bumchul; Kang, Jaehyeon; Doh, Nakju Abstract: Recent progresses in image-based rendering (IBR) have demonstrated the feasibility of photo-realistic modeling in room-scale indoor spaces. However, it is difficult to extend the method to large-scale indoor spaces, because the computational complexity increases exponentially as the geometric complexity increases. In this study, we propose a framework that automatically generates photo-realistic model of large-scale indoor spaces. We first define primary factors that increase geometrical complexity as geometrically excluded objects (GEOs). The proposed framework removes GEOs in images and point clouds to efficiently represent large-scale indoor spaces. To this end, we introduce a segmentation method to segment GEOs from every image coherently. In addition, we also introduce an image inpainting method to fill in the segmented images for photo-realistic indoor modeling. Experiments are conducted in three small-scale spaces and two large-scale indoor spaces. In the experiments, the proposed modules are validated thoroughly. In addition, the experimental results show that the proposed method enables to generate photo-realistic indoor models automatically and efficiently. Tue, 01 Nov 2022 00:00:00 GMT https://scholar.korea.ac.kr/handle/2021.sw.korea/146526 2022-11-01T00:00:00Z https://scholar.korea.ac.kr/handle/2021.sw.korea/145641 Title: Myristoylation-dependent palmitoylation of cyclin Y modulates long-term potentiation and spatial learning Authors: Seo, Jiyeon; Hwang, Hongik; Choi, Yuri; Jung, Sunmin; Hong, Jung-Hwa; Yoon, Bong-June; Rhim, Hyewhon; Park, Mikyoung Abstract: Many psychiatric disorders accompany deficits in cognitive functions and synaptic plasticity, and abnormal lipid modifications of neuronal proteins are associated with their pathophysiology. Lipid modifications, including palmitoylation and myristoylation, play crucial roles in the subcellular localization and trafficking of proteins. Cyclin Y (CCNY), enriched in the postsynaptic compartment, acts as an inhibitory modulator of functional and structural long-term potentiation (LTP) in the hippocampal neurons. However, cellular and molecular mecha-nisms underlying CCNY-mediated inhibitory functions in the synapse remain largely unknown. Here, we report that myristoylation located CCNY to the trans-Golgi network (TGN), and subsequent palmitoylation directed the myristoylated CCNY from the TGN to the synaptic cell surface. This myristoylation-dependent palmitoylation of CCNY was required for the inhibitory role of CCNY in excitatory synaptic transmission, activity-induced dy-namics of AMPA receptors and PSD-95, LTP, and spatial learning. Furthermore, spatial learning significantly reduced palmitoyl-and myristoyl-CCNY levels, indicating that spatial learning lowers the synaptic abundance of CCNY. Our findings provide mechanistic insight into how CCNY is clustered adjacent to postsynaptic sites where it could play its inhibitory roles in synaptic plasticity and spatial learning. Tue, 01 Nov 2022 00:00:00 GMT https://scholar.korea.ac.kr/handle/2021.sw.korea/145641 2022-11-01T00:00:00Z https://scholar.korea.ac.kr/handle/2021.sw.korea/145918 Title: Crystal structure of Ate1, arginyl-tRNA-protein transferase 1 Authors: Song, Hyun Kyu Mon, 31 Oct 2022 00:00:00 GMT https://scholar.korea.ac.kr/handle/2021.sw.korea/145918 2022-10-31T00:00:00Z