Electron Tomography and Synapse StudyElectron Tomography and Synapse Study
- Other Titles
- Electron Tomography and Synapse Study
- Authors
- 김현욱; 김다솜; 유임주
- Issue Date
- 2014
- Publisher
- 한국현미경학회
- Keywords
- Synapse; Neuroscience; Synaptic vesicle; Pooling; Postsynaptic density
- Citation
- 한국현미경학회지, v.44, no.3, pp.83 - 87
- Indexed
- KCI
- Journal Title
- 한국현미경학회지
- Volume
- 44
- Number
- 3
- Start Page
- 83
- End Page
- 87
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/100640
- ISSN
- 2287-5123
- Abstract
- Electron tomography (ET) is a useful tool to investigate three-dimensional details based on virtual slices of relative thick specimen, and it requires complicated procedures consisted of image acquisition steps and image processing steps with computer program. Although the complicated step, this technique allows us to overcome some limitations of conventional transmission electron microscopy: (1) overlapping of information in the ultrathin section covering from 30 nm to 90 nm when we observe very small structures, (2) fragmentation of the information when we study larger structures over 100 nm. There are remarkable biological findings with ET, especially in the field of neuroscience, although it is not popular yet. Understanding of behavior of synaptic vesicle, active zone, pooling and fusion in the presynaptic terminal have been enhanced thanks to ET. Some sophisticated models of postsynaptic density with ET and immune labeling are introduced recently. In this review, we introduce principles, practical steps of ET and some recent researches in synapse biology.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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