Antifungal activity of Rheum undulatum on Candida albicans by the changes in membrane permeability
- Authors
- Lee, H.-S.; Kim, Y.
- Issue Date
- 2014
- Publisher
- Microbiological Society of Korea
- Keywords
- Antifungal activity; Biofilm; Candida albicans; Membrane permeability; Rheum undulatum
- Citation
- Korean Journal of Microbiology, v.50, no.4, pp.360 - 367
- Indexed
- SCOPUS
KCI
- Journal Title
- Korean Journal of Microbiology
- Volume
- 50
- Number
- 4
- Start Page
- 360
- End Page
- 367
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/100683
- DOI
- 10.7845/kjm.2014.4085
- ISSN
- 0440-2413
- Abstract
- Candida albicans is an opportunistic and the most prevalent fungal pathogen that can cause superficial and systemic infections in immunocompromised patients. C. albicans can promote the transition from budding yeast to filamentous form, generating biofilms. Infections associated with C. albicans biofilms are frequently resistant to conventional antifungal therapy. Therefore, the development of more effective antifungal drugs related with biofilm formation is required urgently. The roots of Rheum undulatum have been used for medicinal purposes in Korea and China traditionally. The aim of present study was to evaluate the effect of R. undulatum extract upon preformed biofilms of 12 clinical C. albicans isolates and the antifungal activities. Its effect on preformed biofilms was evaluated using XTT reduction assay, and metabolic activity of all tested strains was reduced significantly (49.4 ± 6.0%) at 0.098 mg/ml R. undulatum. The R. undulatum extract blocked the adhesion of C. albicans biofilms to polystyrene surfaces, and damaged the cell membrane integrity of C. albicans which was analyzed by CFDA, AM, and propidium iodide double staining. It caused cell lysis which was observed by Confocal laser scanning and phase contrast microscope after propidium iodide and neutral red staining, respectively. Membrane permeability was changed as evidenced by crystal violet uptake. The data suggest that R. undulatum inhibits biofilm formation by C. albicans, which can be associated with the damage of the cell membrane integrity, the changes in the membrane permeability and the cell lysis of C. albicans. © 2014, The Microbiological Society of Korea.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Biotechnology and Bioinformatics > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.