The Effect of Hypertonic Saline on mRNA of Proinflammatory Cytokines in Lipopolysaccharide-Stimulated Polymorphonuclear Cells
DC Field | Value | Language |
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dc.contributor.author | Choi, S.-H. | - |
dc.contributor.author | Yoon, Y.-H. | - |
dc.contributor.author | Kim, J.-Y. | - |
dc.contributor.author | Moon, S.-W. | - |
dc.contributor.author | Cho, Y.-D. | - |
dc.contributor.author | Yeom, J.-W. | - |
dc.date.accessioned | 2021-09-05T16:11:55Z | - |
dc.date.available | 2021-09-05T16:11:55Z | - |
dc.date.created | 2021-06-17 | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0011-393X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/100828 | - |
dc.description.abstract | Background: Hypertonic saline is often used to resuscitate patients experiencing shock. In such conditions, polymorphonuclear cells and Toll-like receptors (TLRs) form an essential part of early induced innate immunity. Objective: To investigate the immunomodulatory effect of hypertonic saline on polymorphonuclear cells by evaluating the changes in TLR-4 receptors and proinflammatory cytokines. Methods: Polymorphonuclear cells were isolated from whole blood using Polymorphprep (Axis-Shield, Oslo, Norway). The isolated polymorphonuclear cells were plated at a density of 1 × 106cells/mL in 6-well flat-bottomed culture plates and were stimulated with 1 μg/mL lipopolysaccharide or N-formyl-methionyl-leucyl-phenylalanine. The stimulated polymorphonuclear cells were cultured in hypertonic saline at 10, 20, or 40 mmol/L above isotonicity. After that, the changes in TLR-4 and cytokines were measured by quantitative real-time polymerase chain reaction and flow cytometry. Results: The level of TLR-4 mRNA expression decreased after stimulation with N-formyl-methionyl-leucyl-phenylalanine, but hypertonic saline did not affect the TLR-4 mRNA expression. TLR-4 mRNA expression was clearly induced upon stimulation with lipopolysaccharide, and the addition of hypertonic saline restored TLR-4 mRNA expression in polymorphonuclear cells. The interleukin-1β mRNA expression was decreased in the hypertonic environment. On the other hand, the tumor necrosis factor-α value was not influenced by the addition of hypertonic saline. Conclusions: Hypertonic saline has an immunomodulatory effect on polymorphonuclear cells through the TLR-4 pathway, and the interleukin-1β-associated pathway is influenced more by hypertonic saline than is the tumor necrosis factor-α-associated pathway. © 2014 The Authors. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | Excerpta Medica Inc. | - |
dc.subject | cytokine | - |
dc.subject | formylmethionylleucylphenylalanine | - |
dc.subject | interleukin 1beta | - |
dc.subject | lipopolysaccharide | - |
dc.subject | messenger RNA | - |
dc.subject | sodium chloride | - |
dc.subject | toll like receptor 4 | - |
dc.subject | tumor necrosis factor alpha | - |
dc.subject | Article | - |
dc.subject | cell density | - |
dc.subject | cell isolation | - |
dc.subject | cell stimulation | - |
dc.subject | controlled study | - |
dc.subject | flow cytometry | - |
dc.subject | gene expression | - |
dc.subject | human | - |
dc.subject | human cell | - |
dc.subject | human cell culture | - |
dc.subject | immunomodulation | - |
dc.subject | normal human | - |
dc.subject | polymorphonuclear cell | - |
dc.subject | priority journal | - |
dc.subject | quantitative assay | - |
dc.subject | real time polymerase chain reaction | - |
dc.title | The Effect of Hypertonic Saline on mRNA of Proinflammatory Cytokines in Lipopolysaccharide-Stimulated Polymorphonuclear Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, S.-H. | - |
dc.contributor.affiliatedAuthor | Cho, Y.-D. | - |
dc.identifier.doi | 10.1016/j.curtheres.2014.06.003 | - |
dc.identifier.scopusid | 2-s2.0-84930667668 | - |
dc.identifier.bibliographicCitation | Current Therapeutic Research - Clinical and Experimental, v.76, pp.58 - 62 | - |
dc.relation.isPartOf | Current Therapeutic Research - Clinical and Experimental | - |
dc.citation.title | Current Therapeutic Research - Clinical and Experimental | - |
dc.citation.volume | 76 | - |
dc.citation.startPage | 58 | - |
dc.citation.endPage | 62 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | cytokine | - |
dc.subject.keywordPlus | formylmethionylleucylphenylalanine | - |
dc.subject.keywordPlus | interleukin 1beta | - |
dc.subject.keywordPlus | lipopolysaccharide | - |
dc.subject.keywordPlus | messenger RNA | - |
dc.subject.keywordPlus | sodium chloride | - |
dc.subject.keywordPlus | toll like receptor 4 | - |
dc.subject.keywordPlus | tumor necrosis factor alpha | - |
dc.subject.keywordPlus | Article | - |
dc.subject.keywordPlus | cell density | - |
dc.subject.keywordPlus | cell isolation | - |
dc.subject.keywordPlus | cell stimulation | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | flow cytometry | - |
dc.subject.keywordPlus | gene expression | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | human cell culture | - |
dc.subject.keywordPlus | immunomodulation | - |
dc.subject.keywordPlus | normal human | - |
dc.subject.keywordPlus | polymorphonuclear cell | - |
dc.subject.keywordPlus | priority journal | - |
dc.subject.keywordPlus | quantitative assay | - |
dc.subject.keywordPlus | real time polymerase chain reaction | - |
dc.subject.keywordAuthor | Cytokine | - |
dc.subject.keywordAuthor | Hypertonic saline solution | - |
dc.subject.keywordAuthor | Immune | - |
dc.subject.keywordAuthor | Inflammation | - |
dc.subject.keywordAuthor | Neutrophil | - |
dc.subject.keywordAuthor | Toll-like receptor 4 | - |
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