Cation-selective electropreconcentration
DC Field | Value | Language |
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dc.contributor.author | Shin, Il Hyung | - |
dc.contributor.author | Kim, Ki-jung | - |
dc.contributor.author | Kim, Jiman | - |
dc.contributor.author | Kim, Hee Chan | - |
dc.contributor.author | Chun, Honggu | - |
dc.date.accessioned | 2021-09-05T17:47:38Z | - |
dc.date.available | 2021-09-05T17:47:38Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1473-0197 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/101244 | - |
dc.description.abstract | A cation-selective microfluidic sample preconcentration system is described. The cation sample was electropreconcentrated using a reversed-direction electroosmotic flow (EOF) and an anion-permselective filter, where an electric double layer (EDL) overlap condition existed. The anion-permselective filter between microchannels was fabricated by three different methods: 1) extending a positively charged, nanoporous, polymer membrane by photopolymerization of poly(diallyldimethylammonium chloride) (PDADMAC); 2) etching a nanochannel and then coating it with a positively-charged monomer, N-[3(trimethoxysilyl)propyl]-N'-(4-vinylbenzyl)ethylenediamine hydrochloride (TMSVE); and, 3) etching a nanochannel and then coating it with a positively-charged, pre-formed polymer, polyE-323. The EOF direction in the microchannel was reversed by both TMSVE and polyE-323 coatings. The cation-selective preconcentration was investigated using charged fluorescent dyes and tetramethylrhodamine isothiocyanate (TRITC)-tagged peptides/proteins. The preconcentration in the three different systems was compared with respect to efficiency, dependence on buffer concentration and pH, tolerable flow rate, and sample adsorption. Both TMSVE- and polyE-323-coated nanochannels showed robust preconcentration at high flow rates, whereas the PDADMAC membrane maintained anion- permselectivity at higher buffer concentrations. The TMSVE-coated nanochannels showed a more stable preconcentration process, whereas the polyE-323-coated nanochannels showed a lower peptide sample adsorption and robust efficiency under a wide range of buffer pHs. The system described here can potentially be used for the preconcentration of cationic peptides/proteins on microfluidic devices for subsequent analyses. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | IONIZATION MASS-SPECTROMETRY | - |
dc.subject | CAPILLARY-ELECTROPHORESIS | - |
dc.subject | SAMPLE PRECONCENTRATION | - |
dc.subject | CONCENTRATION POLARIZATION | - |
dc.subject | MICROFLUIDIC DEVICES | - |
dc.subject | CHIP | - |
dc.subject | PROTEINS | - |
dc.subject | FLOW | - |
dc.subject | MICROCHIP | - |
dc.subject | STACKING | - |
dc.title | Cation-selective electropreconcentration | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chun, Honggu | - |
dc.identifier.doi | 10.1039/c4lc00024b | - |
dc.identifier.scopusid | 2-s2.0-84899841355 | - |
dc.identifier.wosid | 000335925400003 | - |
dc.identifier.bibliographicCitation | LAB ON A CHIP, v.14, no.11, pp.1811 - 1815 | - |
dc.relation.isPartOf | LAB ON A CHIP | - |
dc.citation.title | LAB ON A CHIP | - |
dc.citation.volume | 14 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1811 | - |
dc.citation.endPage | 1815 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.subject.keywordPlus | IONIZATION MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | CAPILLARY-ELECTROPHORESIS | - |
dc.subject.keywordPlus | SAMPLE PRECONCENTRATION | - |
dc.subject.keywordPlus | CONCENTRATION POLARIZATION | - |
dc.subject.keywordPlus | MICROFLUIDIC DEVICES | - |
dc.subject.keywordPlus | CHIP | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | FLOW | - |
dc.subject.keywordPlus | MICROCHIP | - |
dc.subject.keywordPlus | STACKING | - |
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