Bimolecular Fluorescence Complementation; Lighting-Up Tau-Tau Interaction in Living Cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tak, HyeJin | - |
dc.contributor.author | Haque, Md Mamunul | - |
dc.contributor.author | Kim, Min Jung | - |
dc.contributor.author | Lee, Joo Hyun | - |
dc.contributor.author | Baik, Ja-Hyun | - |
dc.contributor.author | Kim, YoungSoo | - |
dc.contributor.author | Kim, Dong Jin | - |
dc.contributor.author | Grailhe, Regis | - |
dc.contributor.author | Kim, Yun Kyung | - |
dc.date.accessioned | 2021-09-05T18:04:35Z | - |
dc.date.available | 2021-09-05T18:04:35Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2013-12-02 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/101322 | - |
dc.description.abstract | Abnormal tau aggregation is a pathological hallmark of many neurodegenerative disorders and it is becoming apparent that soluble tau aggregates play a key role in neurodegeneration and memory impairment. Despite this pathological importance, there is currently no single method that allows monitoring soluble tau species in living cells. In this regard, we developed a cell-based sensor that visualizes tau self-assembly. By introducing bimolecular fluorescence complementation (BiFC) technique to tau, we were able to achieve spatial and temporal resolution of tau-tau interactions in a range of states, from soluble dimers to large aggregates. Under basal conditions, tau-BiFC cells exhibited little fluorescence intensity, implying that the majority of tau molecules exist as monomers. Upon chemically induced tau hyperphosphorylation, BiFC fluorescence greatly increased, indicating an increased level of tau-tau interactions. As an indicator of tau assembly, our BiFC sensor would be a useful tool for investigating tau pathology. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.subject | NEURITE OUTGROWTH | - |
dc.subject | PROTEIN-KINASE | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | MICROTUBULES | - |
dc.subject | AGGREGATION | - |
dc.subject | ACTIVATION | - |
dc.subject | BINDING | - |
dc.subject | ASSAY | - |
dc.subject | NEURODEGENERATION | - |
dc.subject | FORSKOLIN | - |
dc.title | Bimolecular Fluorescence Complementation; Lighting-Up Tau-Tau Interaction in Living Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Baik, Ja-Hyun | - |
dc.identifier.doi | 10.1371/journal.pone.0081682 | - |
dc.identifier.scopusid | 2-s2.0-84891427788 | - |
dc.identifier.wosid | 000327944500087 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.8, no.12 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 8 | - |
dc.citation.number | 12 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | NEURITE OUTGROWTH | - |
dc.subject.keywordPlus | PROTEIN-KINASE | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | MICROTUBULES | - |
dc.subject.keywordPlus | AGGREGATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | NEURODEGENERATION | - |
dc.subject.keywordPlus | FORSKOLIN | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.