Tiam-1, a GEF for Rac1, plays a critical role in metformin-mediated glucose uptake in C2C12 cells
DC Field | Value | Language |
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dc.contributor.author | You, Ga Young | - |
dc.contributor.author | Lee, Jung Ok | - |
dc.contributor.author | Kim, Ji Hae | - |
dc.contributor.author | Kim, Nami | - |
dc.contributor.author | Lee, Soo Kyung | - |
dc.contributor.author | Moon, Ji Wook | - |
dc.contributor.author | Jie, Sha | - |
dc.contributor.author | Lee, Hye Jeong | - |
dc.contributor.author | Kim, Su Jin | - |
dc.contributor.author | Park, Sun Hwa | - |
dc.contributor.author | Kim, Hyeon Soo | - |
dc.date.accessioned | 2021-09-05T18:09:55Z | - |
dc.date.available | 2021-09-05T18:09:55Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2013-12 | - |
dc.identifier.issn | 0898-6568 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/101351 | - |
dc.description.abstract | Metformin is known to stimulate glucose uptake, but the mechanism for this action is not fully understood. In this study, AMPK activators (AICAR and metformin) increased the expression of T-lymphoma invasion and metastasis-inducing protein-1 (Tiam-1), a Rac1 specific guanine nucleotide exchange factor (GEF), mRNA and protein in skeletal muscle C2C12 cells. Metformin increases the serine-phosphorylation of Tiam-1 by AMPK and induces interaction between Tiam-1 and 14-3-3. Pharmacologic inhibition of AMPK blocks this interaction, indicating that 14-3-3 may be required for induction of Tiam-1 by AMPK. Metformin also increases the phosphorylation of p21-activated kinase 1 (PAK1), a direct downstream target of Rac1, dependent on AMPK. Tiam-1 is down-regulated at high glucose concentrations in cultured cells and in the db/db mouse model of hyperglycemia. Furthermore, Tiam-1 knock-down blocked metformin-induced increase in glucose uptake. These findings suggest that metformin promotes cellular glucose uptake in part through Tiam-1 induction. (C) 2013 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.subject | ACTIVATED PROTEIN-KINASE | - |
dc.subject | SMALL GTPASE RAC1 | - |
dc.subject | SKELETAL-MUSCLE | - |
dc.subject | INSULIN-RESISTANCE | - |
dc.subject | GLUT4 TRANSLOCATION | - |
dc.subject | FATTY-ACID | - |
dc.subject | AMP KINASE | - |
dc.subject | ACTIN | - |
dc.subject | TRANSPORT | - |
dc.subject | GLUCOSE-TRANSPORTER-4 | - |
dc.title | Tiam-1, a GEF for Rac1, plays a critical role in metformin-mediated glucose uptake in C2C12 cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Sun Hwa | - |
dc.contributor.affiliatedAuthor | Kim, Hyeon Soo | - |
dc.identifier.doi | 10.1016/j.cellsig.2013.08.018 | - |
dc.identifier.scopusid | 2-s2.0-84884388032 | - |
dc.identifier.wosid | 000328179800022 | - |
dc.identifier.bibliographicCitation | CELLULAR SIGNALLING, v.25, no.12, pp.2558 - 2565 | - |
dc.relation.isPartOf | CELLULAR SIGNALLING | - |
dc.citation.title | CELLULAR SIGNALLING | - |
dc.citation.volume | 25 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 2558 | - |
dc.citation.endPage | 2565 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | SMALL GTPASE RAC1 | - |
dc.subject.keywordPlus | SKELETAL-MUSCLE | - |
dc.subject.keywordPlus | INSULIN-RESISTANCE | - |
dc.subject.keywordPlus | GLUT4 TRANSLOCATION | - |
dc.subject.keywordPlus | FATTY-ACID | - |
dc.subject.keywordPlus | AMP KINASE | - |
dc.subject.keywordPlus | ACTIN | - |
dc.subject.keywordPlus | TRANSPORT | - |
dc.subject.keywordPlus | GLUCOSE-TRANSPORTER-4 | - |
dc.subject.keywordAuthor | AMPK | - |
dc.subject.keywordAuthor | GEF | - |
dc.subject.keywordAuthor | Glucose uptake | - |
dc.subject.keywordAuthor | PAK1 | - |
dc.subject.keywordAuthor | Tiam-1 | - |
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