Antimicrobial activities of Eugenia caryophyllata extract and its major chemical constituent eugenol against Streptococcus pneumoniae
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yadav, Mukesh Kumar | - |
dc.contributor.author | Park, Seok-Won | - |
dc.contributor.author | Chae, Sung-Won | - |
dc.contributor.author | Song, Jae-Jun | - |
dc.contributor.author | Kim, Ho Chul | - |
dc.date.accessioned | 2021-09-05T18:11:25Z | - |
dc.date.available | 2021-09-05T18:11:25Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2013-12 | - |
dc.identifier.issn | 0903-4641 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/101361 | - |
dc.description.abstract | In this study, we investigate the antimicrobial activities of both Eugenia caryophyllata (Ec) extract and its major component eugenol (4-allyl-2-methoxyphenol) against Streptococcus pneumoniae. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by microdilution method. Pneumococcal biofilms were detected by crystal-violet microtiter plate assay, followed by colony-forming unit counts and visualized by scanning electron microscope (SEM). The synergistic effect of eugenol and penicillin was determined by checker-board method. Both the eugenol and the Ec extract inhibited pneumococcal growth in a concentration-dependent manner. The MIC and MBC of eugenol were 0.06% and 0.12%, respectively. Eugenol at a concentration of 0.12% completely killed S. pneumoniae within 60min of exposure. The kill rate of planktonic cells was most rapid during the first 15min of contact with eugenol. The addition of eugenol or Ec extract inhibited in vitro biofilm formation. In already established biofilms, the inhibitory effect of eugenol or Ec extract was more significant in terms of cell viability than in terms of disruption of the biofilm matrix. SEM analysis revealed non-viable and disruptive action of eugenol on the cell membrane of bacteria of biofilms. It was found that eugenol and penicillin produced a synergistic effect against S. pneumoniae. In conclusion, eugenol and Ec extract efficiently inhibited S. pneumoniae in planktonic growth and within biofilms. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | CLOVE ESSENTIAL OIL | - |
dc.subject | BIOFILM FORMATION | - |
dc.subject | ANTIOXIDANT PROPERTIES | - |
dc.subject | STAPHYLOCOCCUS-AUREUS | - |
dc.subject | ANTIFUNGAL ACTIVITY | - |
dc.subject | RESPIRATORY-TRACT | - |
dc.subject | IN-VITRO | - |
dc.subject | EAR | - |
dc.subject | ANTIBIOTICS | - |
dc.subject | INFECTIONS | - |
dc.title | Antimicrobial activities of Eugenia caryophyllata extract and its major chemical constituent eugenol against Streptococcus pneumoniae | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chae, Sung-Won | - |
dc.identifier.doi | 10.1111/apm.12067 | - |
dc.identifier.scopusid | 2-s2.0-84888429898 | - |
dc.identifier.wosid | 000327311700011 | - |
dc.identifier.bibliographicCitation | APMIS, v.121, no.12, pp.1198 - 1206 | - |
dc.relation.isPartOf | APMIS | - |
dc.citation.title | APMIS | - |
dc.citation.volume | 121 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1198 | - |
dc.citation.endPage | 1206 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalResearchArea | Pathology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Pathology | - |
dc.subject.keywordPlus | CLOVE ESSENTIAL OIL | - |
dc.subject.keywordPlus | BIOFILM FORMATION | - |
dc.subject.keywordPlus | ANTIOXIDANT PROPERTIES | - |
dc.subject.keywordPlus | STAPHYLOCOCCUS-AUREUS | - |
dc.subject.keywordPlus | ANTIFUNGAL ACTIVITY | - |
dc.subject.keywordPlus | RESPIRATORY-TRACT | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | EAR | - |
dc.subject.keywordPlus | ANTIBIOTICS | - |
dc.subject.keywordPlus | INFECTIONS | - |
dc.subject.keywordAuthor | Eugenia caryophyllata | - |
dc.subject.keywordAuthor | eugenol | - |
dc.subject.keywordAuthor | biofilms | - |
dc.subject.keywordAuthor | antibacterial | - |
dc.subject.keywordAuthor | Streptococcus pneumoniae | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.