Chloroform induces HIF-1 alpha-dependent VEGF expression in human keratinocytes
- Authors
- Nam, Jae Jun; Lee, Hana; Bae, Hyun Cheol; Kim, Jinhee; Jeong, Sang Hoon; Ryu, Woo-In; Son, Sang Wook
- Issue Date
- 12월-2013
- Publisher
- KOREAN SOCIETY TOXICOGENOMICS & TOXICOPROTEOMICS-KSTT
- Keywords
- Vascular endothelial growth factor; Hypoxia-inducible factor-1 alpha; Chloroform; Human keratinocytes
- Citation
- MOLECULAR & CELLULAR TOXICOLOGY, v.9, no.4, pp.335 - 340
- Indexed
- SCIE
SCOPUS
KCI
OTHER
- Journal Title
- MOLECULAR & CELLULAR TOXICOLOGY
- Volume
- 9
- Number
- 4
- Start Page
- 335
- End Page
- 340
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/101515
- DOI
- 10.1007/s13273-013-0042-z
- ISSN
- 1738-642X
- Abstract
- Vascular endothelial growth factor (VEGF), a primary angiogenic factor, can be expressed under various pathological conditions. We showed that VEGF was upregulated when human keratinocytes were treated with the volatile organic compound chloroform. We investigated the regulation of VEGF expression by the transcription factor hypoxia-inducible factor-1 alpha (HIF-1 alpha). HIF-1 alpha, a protein that normally mediates the expression of proteins in hypoxia, controlled VEGF expression in human keratinocytes treated with chloroform. No significant change was seen in HIF-1 alpha mRNA level, consistent with previous reports that HIF-1 alpha protein is constitutively expressed and degraded. Furthermore, transfection of cells with plasmids that overexpressed HIF-1 alpha upregulated VEGF expression. Small interfering RNA against HIF-1 alpha suppressed chloroform-induced VEGF expression in keratinocytes, indicating that HIF-1 alpha was involved in VEGF expression. Thus, the results of this study indicated that HIF-1 alpha mediated the expression of VEGF in human keratinocytes treated with chloroform.
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Collections - Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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