ZnO nanoparticles induce TNF-alpha expression via ROS-ERK-Egr-1 pathway in human keratinocytes
DC Field | Value | Language |
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dc.contributor.author | Jeong, Sang Hoon | - |
dc.contributor.author | Kim, Hee Joo | - |
dc.contributor.author | Ryu, Hwa Jeong | - |
dc.contributor.author | Ryu, Woo In | - |
dc.contributor.author | Park, Yoon-Hee | - |
dc.contributor.author | Bae, Hyun Cheol | - |
dc.contributor.author | Jang, Yeon Sue | - |
dc.contributor.author | Son, Sang Wook | - |
dc.date.accessioned | 2021-09-05T18:46:53Z | - |
dc.date.available | 2021-09-05T18:46:53Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2013-12 | - |
dc.identifier.issn | 0923-1811 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/101528 | - |
dc.description.abstract | Background: The area of nanotechnology continues to expand rapidly and zinc oxide (ZnO) nanoparticles (NPs) are widely being used in cosmetics and sunscreens. Although ZnO-NPs are considered materials that can potentially cause skin inflammation, the underlying mechanisms remain elusive. Objective: The aim of this study was to investigate the signaling pathways of a cutaneous inflammatory response induced by ZnO-NPs. ZnO-NPs increased the early growth response-1 (Egr-1) expression, promoter activity and its nuclear translocation in HaCaT cells. Methods: HaCaT cells and primary keratinocytes were exposed to ZnO NPs over a range of doses and time course. Protein levels and mRNA levels of Egr-1 and mitogen-activated protein kinase (MAPK) were measured by Western blot and ELISA, respectively. As an in vivo study, ZnO-NPs were applicated on mouse skin, and immunohistochemical stain with TNE-alpha and Egr-1 was done. Results: ZnO-NPs activated extracellular signal-regulated kinase (ERK) of MAPK pathways. The upregulation of Egr-1 expression by ZnO-NPs stimulation was found to be inhibited by an ERK inhibitor, but by neither c-Jun-N-terminal kinase (JNK) nor p38 inhibitor. Antioxidative N-acetyl-cysteine (NAC) strongly inhibited the level of Egr-1 and phosphorylated ERR expression in ZnO-NPs treated cells. ZnO NPs also increased tumor necrosis factor (TNE)-alpha expression and secretion, which were inhibited by the blockade of Egr-1 expression. Conclusions: The present study demonstrated that ZnO-NPs might induce inflammatory response via ROS-ERK-Egr-1 pathway in human keratinocytes. (C) 2013 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.subject | TITANIUM-DIOXIDE NANOPARTICLES | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | ZINC-OXIDE | - |
dc.subject | CIGARETTE-SMOKE | - |
dc.subject | TRANSCRIPTION FACTOR | - |
dc.subject | TIO2 NANOPARTICLES | - |
dc.subject | SKIN PENETRATION | - |
dc.subject | DNA-DAMAGE | - |
dc.subject | IN-VIVO | - |
dc.subject | GROWTH | - |
dc.title | ZnO nanoparticles induce TNF-alpha expression via ROS-ERK-Egr-1 pathway in human keratinocytes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ryu, Hwa Jeong | - |
dc.contributor.affiliatedAuthor | Son, Sang Wook | - |
dc.identifier.doi | 10.1016/j.jdermsci.2013.08.002 | - |
dc.identifier.scopusid | 2-s2.0-84887196378 | - |
dc.identifier.wosid | 000327919800008 | - |
dc.identifier.bibliographicCitation | JOURNAL OF DERMATOLOGICAL SCIENCE, v.72, no.3, pp.263 - 273 | - |
dc.relation.isPartOf | JOURNAL OF DERMATOLOGICAL SCIENCE | - |
dc.citation.title | JOURNAL OF DERMATOLOGICAL SCIENCE | - |
dc.citation.volume | 72 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 263 | - |
dc.citation.endPage | 273 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Dermatology | - |
dc.relation.journalWebOfScienceCategory | Dermatology | - |
dc.subject.keywordPlus | TITANIUM-DIOXIDE NANOPARTICLES | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | ZINC-OXIDE | - |
dc.subject.keywordPlus | CIGARETTE-SMOKE | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | TIO2 NANOPARTICLES | - |
dc.subject.keywordPlus | SKIN PENETRATION | - |
dc.subject.keywordPlus | DNA-DAMAGE | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordAuthor | Egr-1 | - |
dc.subject.keywordAuthor | ERK | - |
dc.subject.keywordAuthor | ZnO nanoparticles | - |
dc.subject.keywordAuthor | Nanotoxicity | - |
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