Neural stem cells inhibit melanin production by activation of Wnt inhibitors
DC Field | Value | Language |
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dc.contributor.author | Hwang, Insik | - |
dc.contributor.author | Park, Ju-Hwang | - |
dc.contributor.author | Park, Hang-Soo | - |
dc.contributor.author | Choi, Kyung-Ah | - |
dc.contributor.author | Seol, Ki-Cheon | - |
dc.contributor.author | Oh, Seung-Ick | - |
dc.contributor.author | Kang, Seongman | - |
dc.contributor.author | Hong, Sunghoi | - |
dc.date.accessioned | 2021-09-05T18:47:15Z | - |
dc.date.available | 2021-09-05T18:47:15Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2013-12 | - |
dc.identifier.issn | 0923-1811 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/101530 | - |
dc.description.abstract | Background: Melanin for skin pigmentation is synthesized from tyrosine via an enzymatic cascade that is controlled by tyrosinase (TYR), tyrosinase-related protein 1 (TRP1), and dopachrome tautomerase/tyrosinase related protein 2 (Dct/TRP2), which are the targets of microphthalmia-associated transcription factor (MITF). MITF is a master regulator of pigmentation and a target of beta-catenin in Wnt/beta-catenin signaling during melanocyte differentiation. Stem cells have been used in skin pigmentation studies, but the mechanisms were not determined for the conditioned medium (CM)-mediated effects. Objectives: In this study, the inhibition and mechanisms of melanin synthesis were elucidated in B16 melanoma cells and UV-B irradiated C57/BL-6 mice that were treated with human neural stem cell-conditioned medium (NSC-CM). Methods: B16-F10 melanoma cells (1.5 x 10(4) cells/well) and the shaved dorsal skin of mice were pretreated with various amount (5, 10, 20, 50, and 100%) of NSC-CM. Melanin contents and TYR activity were measured by a Spectramax spectrophotometer. The expression of TYR, TRP1, Dct/TRP2, MITE, beta-catenin and Wnt inhibitors were evaluated by RT-PCR and western blot. The dorsal skin samples were analyzed by immunofluorescence with various antibodies and compared with that control of tissues. Results: Marked decreases were evident in melanin content and TYR, TRP1, DCT/TRP2, MITF, and beta-catenin expression in B16 cells and C57/BL-6 mice. NSC-CM negatively regulated Wnt/beta-catenin signaling by decreasing the expression of beta-catenin protein, which resulted from robust expression of Wnt inhibitors Dickkopf-1 (DKK1) and secreted frizzled-related protein 2 (sFRP2). Conclusions: These results demonstrate that NSC-CM suppresses melanin production in vitro and in vivo, suggesting that factors in NSC-CM may play an important role in deregulation of epidermal melanogenesis. (C) 2013 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.subject | MICROPHTHALMIA GENE-PRODUCT | - |
dc.subject | HUMAN DERMAL FIBROBLASTS | - |
dc.subject | TRANSCRIPTION FACTOR | - |
dc.subject | BETA-CATENIN | - |
dc.subject | SECRETORY FACTORS | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | CANCER CELLS | - |
dc.subject | IN-VITRO | - |
dc.subject | MITF | - |
dc.subject | DIFFERENTIATION | - |
dc.title | Neural stem cells inhibit melanin production by activation of Wnt inhibitors | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Seung-Ick | - |
dc.contributor.affiliatedAuthor | Kang, Seongman | - |
dc.contributor.affiliatedAuthor | Hong, Sunghoi | - |
dc.identifier.doi | 10.1016/j.jdermsci.2013.08.006 | - |
dc.identifier.scopusid | 2-s2.0-84887159190 | - |
dc.identifier.wosid | 000327919800009 | - |
dc.identifier.bibliographicCitation | JOURNAL OF DERMATOLOGICAL SCIENCE, v.72, no.3, pp.274 - 283 | - |
dc.relation.isPartOf | JOURNAL OF DERMATOLOGICAL SCIENCE | - |
dc.citation.title | JOURNAL OF DERMATOLOGICAL SCIENCE | - |
dc.citation.volume | 72 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 274 | - |
dc.citation.endPage | 283 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Dermatology | - |
dc.relation.journalWebOfScienceCategory | Dermatology | - |
dc.subject.keywordPlus | MICROPHTHALMIA GENE-PRODUCT | - |
dc.subject.keywordPlus | HUMAN DERMAL FIBROBLASTS | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | BETA-CATENIN | - |
dc.subject.keywordPlus | SECRETORY FACTORS | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | CANCER CELLS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | MITF | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordAuthor | Neural stem cell-conditioned medium | - |
dc.subject.keywordAuthor | Melanin | - |
dc.subject.keywordAuthor | Tyrosinase | - |
dc.subject.keywordAuthor | MITF | - |
dc.subject.keywordAuthor | Wnt inhibitors | - |
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