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Polycyclic aromatic hydrocarbon (PAH)-mediated upregulation of hepatic microRNA-181 family promotes cancer cell migration by targeting MAPK phosphatase-5, regulating the activation of p38 MAPK

Authors
Song, Mi-KyungPark, Yong-KeunRyu, Jae-Chun
Issue Date
15-11월-2013
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Keywords
Polycyclic aromatic hydrocarbons (PAHs); Hepatocellular carcinoma (HCC); MicroRNA (miRNA); Migration; MAPK phosphatase (MKP); p38 MAPK
Citation
TOXICOLOGY AND APPLIED PHARMACOLOGY, v.273, no.1, pp.130 - 139
Indexed
SCIE
SCOPUS
Journal Title
TOXICOLOGY AND APPLIED PHARMACOLOGY
Volume
273
Number
1
Start Page
130
End Page
139
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/101606
DOI
10.1016/j.taap.2013.08.016
ISSN
0041-008X
Abstract
Growing evidence indicates that changes in microRNA (miRNA) expression in cancer induced by chemical carcinogens play an important role in cancer development and progression by regulating related genes. However, the mechanisms underlying miRNA involvement in hepatocarcinogenesis induced by polycyclic aromatic hydrocarbons (PAHs) remain unclear. Thus, the identification of aberrant miRNA expression during PAH-induced cancer cell migration will lead to a better understanding of the substantial role of miRNAs in cancer progression. In the present study, miRNA expression profiling showed significant upregulation of miR-181a, -181b, and -181d in human hepatocellular carcinoma cells (HepG2 line) exposed to benzo[a]anthracene (BA) and benzo[k]fluoranthene (BF). MAPK phosphatase-5 (MKP-5), a validated miR-181 target that deactivates MAPKs, was markedly suppressed while phosphorylation of p38 MAPK was increased after BA and BF exposure. The migration of HepG2 cells, observed using the scratch wound-healing assay, also increased in a dose-dependent manner. Depletion of miR-181 family members by miRNA inhibitors enhanced the expression of MKP-5 and suppressed the phosphorylation of p38 MAPK. Furthermore, the depletion of the miR-181 family inhibited cancer cell migration. Based on these results, we conclude that the miR-181 family plays a critical role in PAH-induced hepatocarcinogenesis by targeting MKP-5, resulting in the regulation of p38 MAPK activation. (C) 2013 Published by Elsevier Inc.
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