Masking Nanoparticle Surfaces for Sensitive and Selective Colorimetric Detection of Proteins
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Byung-Ho | - |
dc.contributor.author | Yoon, In Seon | - |
dc.contributor.author | Lee, Jae-Seung | - |
dc.date.accessioned | 2021-09-05T19:15:30Z | - |
dc.date.available | 2021-09-05T19:15:30Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2013-11-05 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/101625 | - |
dc.description.abstract | We have developed a convenient and efficient colorimetric detection system for protein targets using aptamer gold nanoparticle conjugates. We take advantage of the correlation between the catalytic properties and the exposed surface area of the nanoparticles, which is inversely proportional to the amount of the aptamer-bound protein targets. As the concentration of the protein target increases, the nanoparticle surface area becomes more masked, thus increasing the reduction time of 4-nitrophenol for the color change. We also reduce the detection time by either redesigning the aptamer sequences or regulating their density. This detection system is highly selective, discriminating the target protein even at a concentration 1000 times higher than the limit of detection (LOD). Importantly, to the best of our knowledge, the LOD with the unaided eye in this work is the lowest for a colorimetric detection system using lysozyme as a model protein (16 nM). Lysozyme in chicken egg whites is directly analyzed using our detection system, whose results are in excellent agreement with the enzyme-linked immunosorbent assay (ELISA) analysis. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | UNMODIFIED GOLD NANOPARTICLES | - |
dc.subject | CATALYTIC-ACTIVITY | - |
dc.subject | AU NANOPARTICLES | - |
dc.subject | NUCLEIC-ACID | - |
dc.subject | APTAMER | - |
dc.subject | DNA | - |
dc.subject | LYSOZYME | - |
dc.subject | IMMOBILIZATION | - |
dc.subject | ANTIBODIES | - |
dc.subject | REDUCTION | - |
dc.title | Masking Nanoparticle Surfaces for Sensitive and Selective Colorimetric Detection of Proteins | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Jae-Seung | - |
dc.identifier.doi | 10.1021/ac4026169 | - |
dc.identifier.scopusid | 2-s2.0-84887777034 | - |
dc.identifier.wosid | 000326711400080 | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.85, no.21, pp.10542 - 10548 | - |
dc.relation.isPartOf | ANALYTICAL CHEMISTRY | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 85 | - |
dc.citation.number | 21 | - |
dc.citation.startPage | 10542 | - |
dc.citation.endPage | 10548 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | UNMODIFIED GOLD NANOPARTICLES | - |
dc.subject.keywordPlus | CATALYTIC-ACTIVITY | - |
dc.subject.keywordPlus | AU NANOPARTICLES | - |
dc.subject.keywordPlus | NUCLEIC-ACID | - |
dc.subject.keywordPlus | APTAMER | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | LYSOZYME | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | ANTIBODIES | - |
dc.subject.keywordPlus | REDUCTION | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.