A rapid and highly sensitive UPLC-MS/MS method using pre-column derivatization with 2-picolylamine for intravenous and percutaneous pharmacokinetics of valproic acid in rats
DC Field | Value | Language |
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dc.contributor.author | Joo, Kyung-Mi | - |
dc.contributor.author | Choi, Dalwoong | - |
dc.contributor.author | Park, Yang-Hui | - |
dc.contributor.author | Yi, Chang-Geun | - |
dc.contributor.author | Jeong, Hye-Jin | - |
dc.contributor.author | Cho, Jun-Cheol | - |
dc.contributor.author | Lim, Kyung-Min | - |
dc.date.accessioned | 2021-09-05T19:24:53Z | - |
dc.date.available | 2021-09-05T19:24:53Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2013-11-01 | - |
dc.identifier.issn | 1570-0232 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/101647 | - |
dc.description.abstract | A rapid, highly sensitive and specific ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) for the detection of valproic acid (VPA) in rat plasma following the topical application was developed and validated. This method was carried out with pre-column derivatization using 2-picolylamine (PA) which reacts with the carboxylic acid group of VPA. The derivatization was completed in 10 min and the resulting PA-VPA derivative enabled the sensitive detection of VPA in selected reaction monitoring (SRM) mode. Sample preparation was done with simple liquid-liquid extraction and chromatographic separation was achieved within 5 min on a C18 column using a gradient elution with the mobile phase of 2 mM ammonium formate containing 0.1% formic acid and methanol. The standard curves were linear over the concentration range of 0.07-200 mu g/mL with a correlation coefficient higher than 0.99. The limit of detection (LOD) and the lower limit of quantification (LLOQ) was 0.03 and 0.07 mu g/mL, respectively with 100 mu L of plasma sample. The intra- and inter-day precisions were measured to be below 10.7% and accuracies were within the range of 94.1-115.9%. The validated method was successfully applied to the pharmacokinetics of VPA in the rat following topical and intravenous applications. (c) 2013 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | IONIZATION MASS-SPECTROMETRY | - |
dc.subject | HUMAN PLASMA | - |
dc.subject | LIQUID-CHROMATOGRAPHY | - |
dc.subject | GAS-CHROMATOGRAPHY | - |
dc.subject | PHASE MICROEXTRACTION | - |
dc.subject | METABOLISM | - |
dc.subject | QUANTIFICATION | - |
dc.subject | SERUM | - |
dc.subject | DRUG | - |
dc.title | A rapid and highly sensitive UPLC-MS/MS method using pre-column derivatization with 2-picolylamine for intravenous and percutaneous pharmacokinetics of valproic acid in rats | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Dalwoong | - |
dc.identifier.doi | 10.1016/j.jchromb.2013.08.022 | - |
dc.identifier.scopusid | 2-s2.0-84884176634 | - |
dc.identifier.wosid | 000325734800006 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.938, pp.35 - 42 | - |
dc.relation.isPartOf | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | - |
dc.citation.title | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | - |
dc.citation.volume | 938 | - |
dc.citation.startPage | 35 | - |
dc.citation.endPage | 42 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | IONIZATION MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | HUMAN PLASMA | - |
dc.subject.keywordPlus | LIQUID-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | GAS-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | PHASE MICROEXTRACTION | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | SERUM | - |
dc.subject.keywordPlus | DRUG | - |
dc.subject.keywordAuthor | Valproic acid | - |
dc.subject.keywordAuthor | 2-Picolylamine | - |
dc.subject.keywordAuthor | Derivatization | - |
dc.subject.keywordAuthor | UPLC-MS/MS | - |
dc.subject.keywordAuthor | Pharmacokinetics | - |
dc.subject.keywordAuthor | Topical application | - |
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