Remote Postconditioning Attenuates Ischemia/Reperfusion Injury in Rat Skeletal Muscle through Mitochondrial ATP-Sensitive K+ Channel-Dependent Mechanism
- Authors
- Lee, Jung Il; Nha, Kyung Wook; Suh, Jin Soo; Choo, Suk Kyu; Park, Jung Ho; Park, Jong Woong
- Issue Date
- 11월-2013
- Publisher
- THIEME MEDICAL PUBL INC
- Keywords
- Ischemia; reperfusion injury; postconditioning; remote postconditioning; ATP-sensitive K+ channel
- Citation
- JOURNAL OF RECONSTRUCTIVE MICROSURGERY, v.29, no.9, pp.571 - 578
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF RECONSTRUCTIVE MICROSURGERY
- Volume
- 29
- Number
- 9
- Start Page
- 571
- End Page
- 578
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/101725
- DOI
- 10.1055/s-0033-1348037
- ISSN
- 0743-684X
- Abstract
- The authors investigated whether a remote postconditioning (remote post-con) procedure attenuated skeletal muscle ischemia/reperfusion (I/R) injury. We determined the optimal protocol of remote post-con and investigated its mechanism. Ischemia was induced for 3 hours in rat left hindlimb and three protocols of remote post-con were applied in right hindlimb just before the end of ischemia. The first (10-second group) involved two cycles of 10 seconds of occlusion followed by 10 seconds of reperfusion. The second (5-minute group) involved two cycles of 5 minutes of occlusion/reperfusion. The third (10-minute group) involved two cycles of 10 minutes of occlusion/reperfusion. In 5- and 10-minute groups, wet/dry ratio and muscle fiber edema were significantly lower than control group. Muscle contractility was preserved in 5- and 10-minute groups. An injection of 5-hydroxydecanoate (a specific blocker of mitochondrial ATP-sensitive K+ [mK(ATP)] channels) impaired this effect. This study demonstrates that remote post-con preserves muscle contractility and reduces tissue edema and necrosis, possibly through the activation of mK(ATP) channels. We suggest that two cycles of 5 minutes of occlusion followed by 5 minutes of reperfusion are optimal protocols of remote post-con in skeletal muscle I/R injury.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
- Graduate School > Department of Biomedical Sciences > 1. Journal Articles
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