Expression, crystallization and preliminary X-ray crystallographic studies of SCP3 coiled-coil domain
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seo, Eun Kyoung | - |
dc.contributor.author | Kim, Tae Woo | - |
dc.contributor.author | Park, Hyun Ho | - |
dc.date.accessioned | 2021-09-05T19:44:47Z | - |
dc.date.available | 2021-09-05T19:44:47Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2013-11 | - |
dc.identifier.issn | 2053-230X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/101765 | - |
dc.description.abstract | The synaptonemal complex protein SCP3 is one of the components of the lateral element of the synaptonemal complex, which is a meiosis-specific complex structure formed at the synapse of homologous chromosomes. In this study, a C-terminal coiled-coil domain, SCP3, was overexpressed in Escherichia coli with an engineered C-terminal His tag. The coiled-coil domain of SCP3 was then purified to homogeneity and crystallized at 293 K. X-ray diffraction data were collected to a resolution of 3.2 angstrom from a crystal belonging to space group C2, with unit-cell parameters a = 121.29, b = 43.08, c = 57.42 angstrom, alpha = 100.71 degrees. The asymmetric unit was estimated to contain three molecules. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | INT UNION CRYSTALLOGRAPHY | - |
dc.subject | SYNAPTONEMAL COMPLEX | - |
dc.subject | CRYSTAL-STRUCTURE | - |
dc.subject | GENETICS | - |
dc.title | Expression, crystallization and preliminary X-ray crystallographic studies of SCP3 coiled-coil domain | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Tae Woo | - |
dc.identifier.doi | 10.1107/S1744309113026663 | - |
dc.identifier.scopusid | 2-s2.0-84887263151 | - |
dc.identifier.wosid | 000326569300023 | - |
dc.identifier.bibliographicCitation | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, v.69, pp.1281 - 1283 | - |
dc.relation.isPartOf | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | - |
dc.citation.title | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | - |
dc.citation.volume | 69 | - |
dc.citation.startPage | 1281 | - |
dc.citation.endPage | 1283 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Crystallography | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Crystallography | - |
dc.subject.keywordPlus | SYNAPTONEMAL COMPLEX | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | GENETICS | - |
dc.subject.keywordAuthor | Coiled-coil domain | - |
dc.subject.keywordAuthor | Synaptonemal complex protein SCP3 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.