Amplification of Resonant Rayleigh Light Scattering Response Using Immunogold Colloids for Detection of Lysozyme
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Phuoc Long Truong | - |
dc.contributor.author | Choi, Seung Phill | - |
dc.contributor.author | Sim, Sang Jun | - |
dc.date.accessioned | 2021-09-05T20:04:38Z | - |
dc.date.available | 2021-09-05T20:04:38Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2013-10-25 | - |
dc.identifier.issn | 1613-6810 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/101846 | - |
dc.description.abstract | A strategy for attomolar-level detection of small molecule-size proteins is reported based on Rayleigh light scattering spectroscopy of individual nanoplasmonic aptasensors by exploiting the outstanding characteristics of gold colloids to amplify the nontransparent resonant signal at ultralow analyte concentrations. The fabrication method utilizes thiol-mediated adsorption of a DNA aptamer on the immobilized Au nanoparticle surface, the interfacial binding characteristics of the aptamer with its target molecules, and the antibody-antigen interaction through plasmonic resonance coupling of the Au nanoparticles. Using lysozyme as a model analyte for disease detection, the detection limit of the aptasensor is approximate to 7 x 10(3) aM, corresponding to the LSPR (max) shift of approximate to 2.25 nm. Up to a 380% increase in the localized resonant (max) shift is demonstrated upon antibody binding to the analyte compared to the primary response during signal amplification using immunogold colloids. This enhancement leads to a limit of detection of approximate to 7 aM, which is an improvement of three orders of magnitude. The results demonstrate substantial promise for developing coupled plasmonic nanostructures for ultrasensitive detection of various biological and chemical analytes. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.subject | SURFACE-PLASMON RESONANCE | - |
dc.subject | GOLD NANOPARTICLES | - |
dc.subject | SINGLE GOLD | - |
dc.subject | SPECTROSCOPY | - |
dc.subject | SENSITIVITY | - |
dc.subject | ADSORPTION | - |
dc.subject | SUBSTRATE | - |
dc.subject | SELECTION | - |
dc.subject | BINDING | - |
dc.subject | NANOROD | - |
dc.title | Amplification of Resonant Rayleigh Light Scattering Response Using Immunogold Colloids for Detection of Lysozyme | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sim, Sang Jun | - |
dc.identifier.doi | 10.1002/smll.201202638 | - |
dc.identifier.scopusid | 2-s2.0-84886099949 | - |
dc.identifier.wosid | 000326017700019 | - |
dc.identifier.bibliographicCitation | SMALL, v.9, no.20, pp.3485 - 3492 | - |
dc.relation.isPartOf | SMALL | - |
dc.citation.title | SMALL | - |
dc.citation.volume | 9 | - |
dc.citation.number | 20 | - |
dc.citation.startPage | 3485 | - |
dc.citation.endPage | 3492 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.relation.journalWebOfScienceCategory | Physics, Condensed Matter | - |
dc.subject.keywordPlus | SURFACE-PLASMON RESONANCE | - |
dc.subject.keywordPlus | GOLD NANOPARTICLES | - |
dc.subject.keywordPlus | SINGLE GOLD | - |
dc.subject.keywordPlus | SPECTROSCOPY | - |
dc.subject.keywordPlus | SENSITIVITY | - |
dc.subject.keywordPlus | ADSORPTION | - |
dc.subject.keywordPlus | SUBSTRATE | - |
dc.subject.keywordPlus | SELECTION | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | NANOROD | - |
dc.subject.keywordAuthor | LSPR | - |
dc.subject.keywordAuthor | rayleigh scattering | - |
dc.subject.keywordAuthor | immunogold colloids | - |
dc.subject.keywordAuthor | plasmon coupling | - |
dc.subject.keywordAuthor | aptasensors | - |
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