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Activation of the Leukotriene B-4 Receptor 2-Reactive Oxygen Species (BLT2-ROS) Cascade following Detachment Confers Anoikis Resistance in Prostate Cancer Cells

Authors
Lee, Jin-WookKim, Jae-Hong
Issue Date
18-10월-2013
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Keywords
Apoptosis; Cancer; Cancer Biology; NADPH Oxidase; Prostate Cancer; Anokis Resistance
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY, v.288, no.42, pp.30054 - 30063
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume
288
Number
42
Start Page
30054
End Page
30063
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/101859
DOI
10.1074/jbc.M113.481283
ISSN
0021-9258
Abstract
Background: Despite the critical role of anoikis resistance in prostate cancer progression, the molecular mechanism causing anoikis resistance remains unclear. Results: BLT2 protects prostate cancer cells against anoikis via a NOX-ROS-NF-B-linked pathway. Conclusion: Activation of the BLT2-linked pathway following cell detachment confers anoikis resistance. Significance: Our findings suggest BLT2 as a key regulator of anoikis resistance in prostate cancer cells. The majority of prostate cancer-related deaths are associated with advanced and metastatic malignancies. Although anoikis resistance has been recognized as one of the hallmarks of metastatic prostate malignancies, the molecular events that cause anoikis resistance are poorly understood. In this study, we found that the detachment of PC-3 prostate cancer cells caused a time-dependent increase in the expression level of the leukotriene B-4 receptor-2 (BLT2) and that BLT2 played a critical role in establishing anoikis resistance in these cells. Blocking BLT2 with the pharmacological inhibitor LY255283 or with RNAi knockdown clearly abolished anoikis resistance and resulted in severe apoptotic death. Additionally, we demonstrated that the activation of NADPH oxidase (NOX) and subsequent generation of reactive oxygen species (ROS) were downstream of BLT2 signaling and led to the activation of NF-B, thus establishing anoikis resistance during cell detachment. Furthermore, we observed that the ectopic expression of BLT2 in normal prostate PWR-1E cells rendered the cells resistant to anoikis and apparently diminished apoptotic cell death following detachment. Taken together, our results suggest that BLT2-NOX-ROS-NF-B cascade induction during detachment confers a novel mechanism of anoikis resistance in prostate cancer cells and potentially contributes to prostate cancer progression.
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생명과학대학 (생명과학부)
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