Interleukin-4 Induces Senescence in Human Renal Carcinoma Cell Lines through STAT6 and p38 MAPK
DC Field | Value | Language |
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dc.contributor.author | Kim, Hag Dong | - |
dc.contributor.author | Yu, Su-Jin | - |
dc.contributor.author | Kim, Hee Suk | - |
dc.contributor.author | Kim, Yong-Jin | - |
dc.contributor.author | Choe, Jeong Min | - |
dc.contributor.author | Park, Yun Gyu | - |
dc.contributor.author | Kim, Joon | - |
dc.contributor.author | Sohn, Jeongwon | - |
dc.date.accessioned | 2021-09-05T20:19:34Z | - |
dc.date.available | 2021-09-05T20:19:34Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2013-10-04 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/101893 | - |
dc.description.abstract | Interleukin (IL)-4, originally identified as a lymphocyte growth factor, can directly inhibit growth of certain tumor cell types. We reported previously that IL-4 induced cell cycle arrest in G(1) phase through an increase in p21(WAF1/CIP1) expression in human renal cell carcinoma (RCC) cell lines. In the present study, we investigated the underlying mechanism of IL-4-induced growth inhibition. In four of six human RCC cell lines, including Caki-1, A498, SNU482, and SNU228, IL-4 induced cellular senescence as demonstrated by enlarged and flattened morphology, increased granularity, and senescence-associated--galactosidase (SA--gal) staining. Signal tranducer and activator of transcription 6 (STAT6) and p38 MAPK were found to mediate IL-4-induced growth inhibition and cellular senescence. Both of these molecules were activated by 10 min after IL-4 treatment, and inhibition of their activity or expression prevented growth suppression and cellular senescence induced by IL-4. Inhibiting or silencing either STAT6 or p38 MAPK alone partially reduced the effect of IL-4, whereas inhibiting or silencing both molecules exerted an additive effect and almost completely abrogated the effect of IL-4. Thus STAT6 and p38 MAPK appeared to independently mediate IL-4-induced growth inhibition and cellular senescence. The p21(WAF1/CIP1) up-regulation that accompanied growth inhibition and cellular senescence by IL-4 was also attenuated additively when p38 MAPK and STAT6 were silenced. Taken together, these results show that IL-4 induces cellular senescence through independent signaling pathways involving STAT6 and p38 MAPK in some human RCC cell lines. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.subject | ONCOGENE-INDUCED SENESCENCE | - |
dc.subject | BREAST-CANCER CELLS | - |
dc.subject | GROWTH-INHIBITION | - |
dc.subject | IL-4 RECEPTOR | - |
dc.subject | SIGNALING MECHANISMS | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | IN-VITRO | - |
dc.subject | APOPTOSIS | - |
dc.subject | PATHWAY | - |
dc.subject | ACTIVATION | - |
dc.title | Interleukin-4 Induces Senescence in Human Renal Carcinoma Cell Lines through STAT6 and p38 MAPK | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Yun Gyu | - |
dc.contributor.affiliatedAuthor | Kim, Joon | - |
dc.contributor.affiliatedAuthor | Sohn, Jeongwon | - |
dc.identifier.doi | 10.1074/jbc.M113.499053 | - |
dc.identifier.scopusid | 2-s2.0-84885158864 | - |
dc.identifier.wosid | 000330298800028 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.288, no.40, pp.28743 - 28754 | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 288 | - |
dc.citation.number | 40 | - |
dc.citation.startPage | 28743 | - |
dc.citation.endPage | 28754 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | ONCOGENE-INDUCED SENESCENCE | - |
dc.subject.keywordPlus | BREAST-CANCER CELLS | - |
dc.subject.keywordPlus | GROWTH-INHIBITION | - |
dc.subject.keywordPlus | IL-4 RECEPTOR | - |
dc.subject.keywordPlus | SIGNALING MECHANISMS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordAuthor | Interleukin | - |
dc.subject.keywordAuthor | p38 MAPK | - |
dc.subject.keywordAuthor | Renal Physiology | - |
dc.subject.keywordAuthor | Senescence | - |
dc.subject.keywordAuthor | STAT Transcription Factor | - |
dc.subject.keywordAuthor | IL-4 | - |
dc.subject.keywordAuthor | Renal Cell Carcinoma | - |
dc.subject.keywordAuthor | STAT6 | - |
dc.subject.keywordAuthor | p21WAF1 | - |
dc.subject.keywordAuthor | CIP1 | - |
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