Quantitative Estimation of the Total Sulfide Concentration in Live Tissues by Two-Photon Microscopy
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lim, Chang Su | - |
dc.contributor.author | Das, Sajal Kumar | - |
dc.contributor.author | Yang, Sun Young | - |
dc.contributor.author | Kim, Eun Sun | - |
dc.contributor.author | Chun, Hoon Jai | - |
dc.contributor.author | Cho, Bong Rae | - |
dc.date.accessioned | 2021-09-05T20:22:27Z | - |
dc.date.available | 2021-09-05T20:22:27Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2013-10-01 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/101911 | - |
dc.description.abstract | Hydrogen sulfide (H2S) is a newly recognized transmitter, which protects various organs from oxidative stress. In this article, we report a ratiometric two-photon probe, TFCA, which can be excited by 750 nm femtosecond pulses, shows a 110-fold increase in the intensity ratio upon reaction with HS- and high selectivity for HS- and can visualize the total sulfide ([H2S] + [HS-]) distribution in live tissue by two-photon microscopy (TPM). We also developed a kinetic method to quantitatively estimate the total sulfide concentration ([H2S] + [HS-]) in live tissues. The kinetic method allowed us to measure the observed rate constants (k(obs)) for the sulfide-induced deazidation reaction of TFCA in live cells and tissues using TPM. The total sulfide concentration was calculated by using k(obs) = k(2)[HS-], with the k(2) value determined in HEPES/EtOH (1/1, pH = 7.2), and [H2S]/[HS-] = [H+]/K-2. The total sulfide concentration was found to be nearly zero in HeLa cells and 4-7 mu M in rat colon tissues. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | HYDROGEN-SULFIDE | - |
dc.subject | FLUORESCENT-PROBES | - |
dc.subject | RESOLUTION | - |
dc.subject | MEDIATOR | - |
dc.subject | COPPER | - |
dc.subject | ROLES | - |
dc.subject | H2S | - |
dc.title | Quantitative Estimation of the Total Sulfide Concentration in Live Tissues by Two-Photon Microscopy | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Eun Sun | - |
dc.contributor.affiliatedAuthor | Chun, Hoon Jai | - |
dc.contributor.affiliatedAuthor | Cho, Bong Rae | - |
dc.identifier.doi | 10.1021/ac402042k | - |
dc.identifier.scopusid | 2-s2.0-84885008195 | - |
dc.identifier.wosid | 000330017500055 | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.85, no.19, pp.9288 - 9295 | - |
dc.relation.isPartOf | ANALYTICAL CHEMISTRY | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 85 | - |
dc.citation.number | 19 | - |
dc.citation.startPage | 9288 | - |
dc.citation.endPage | 9295 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | HYDROGEN-SULFIDE | - |
dc.subject.keywordPlus | FLUORESCENT-PROBES | - |
dc.subject.keywordPlus | RESOLUTION | - |
dc.subject.keywordPlus | MEDIATOR | - |
dc.subject.keywordPlus | COPPER | - |
dc.subject.keywordPlus | ROLES | - |
dc.subject.keywordPlus | H2S | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.