Gingival fibroblasts from periodontitis patients exhibit inflammatory characteristics in vitro
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Baek, Keum Jin | - |
dc.contributor.author | Choi, Youngnim | - |
dc.contributor.author | Ji, Suk | - |
dc.date.accessioned | 2021-09-05T20:26:19Z | - |
dc.date.available | 2021-09-05T20:26:19Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2013-10 | - |
dc.identifier.issn | 0003-9969 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/101933 | - |
dc.description.abstract | Objective: Gingival fibroblasts (GFs) are an important regulatory cell type in the progression of periodontitis. This study aimed to compare the expression levels of genes associated with inflammation, extracellular matrix degradation and bone destruction in GFs isolated from healthy and periodontitis subjects in the absence and presence of Porphyromonas gingivalis. Designs: Primary GFs from healthy (n = 10) and periodontitis subjects (n = 10) were stimulated in vitro with viable P. gingivalis ATCC 49417 and 3 clinical isolates of P. gingivalis with type II fimbriae from one healthy subject (KUMC-H1) and two periodontitis patients (KUMC-P1, -P2). The mRNA expression of proinflammatory cytokines (interleukin (IL)-6, IL-8, IL-1B), anti-inflammatory cytokines (IL-4, IL-10), matrix metalloproteinase (MMP)-1 and 2, tissue inhibitor matrix metalloproteinase (TIMP)-3 and osteoprotegerin (OPG) were assessed using real-time PCR. The levels of IL-6, IL-1 beta and TIMP-3 protein were measured by an enzyme-linked immunosorbent assay. Results: The mRNA expression of IL-6, IL-1B and TIMP-3 was higher in the periodontitis group compared with the healthy group, whereas IL-4 expression was higher in the healthy group both in the absence and presence of the P. gingivalis strains. The expression levels of IL-6, IL-10 and TIMP-3 protein were also higher in the periodontitis group in the absence and/or presence of the P. gingivalis strains. There was inter-strain variability among P. gingivalis strains in the ability to induce expression of the proinflammatory cytokines, MMPs and OPG and in the ability to degrade IL-6 protein. Conclusion: High expression of proinflammatory cytokines and TIMP-3 and low expression of IL-4 can be a signature of GFs associated with periodontitis. (C) 2013 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.subject | PORPHYROMONAS-GINGIVALIS | - |
dc.subject | MATRIX METALLOPROTEINASES | - |
dc.subject | FIMA GENOTYPES | - |
dc.subject | SUBGINGIVAL PLAQUE | - |
dc.subject | IMMUNE-RESPONSES | - |
dc.subject | EPITHELIAL-CELLS | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | POLYMORPHISMS | - |
dc.subject | LIGAMENT | - |
dc.subject | STRAINS | - |
dc.title | Gingival fibroblasts from periodontitis patients exhibit inflammatory characteristics in vitro | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ji, Suk | - |
dc.identifier.doi | 10.1016/j.archoralbio.2013.07.007 | - |
dc.identifier.scopusid | 2-s2.0-84888043991 | - |
dc.identifier.wosid | 000325236600003 | - |
dc.identifier.bibliographicCitation | ARCHIVES OF ORAL BIOLOGY, v.58, no.10, pp.1282 - 1292 | - |
dc.relation.isPartOf | ARCHIVES OF ORAL BIOLOGY | - |
dc.citation.title | ARCHIVES OF ORAL BIOLOGY | - |
dc.citation.volume | 58 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1282 | - |
dc.citation.endPage | 1292 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Dentistry, Oral Surgery & Medicine | - |
dc.relation.journalWebOfScienceCategory | Dentistry, Oral Surgery & Medicine | - |
dc.subject.keywordPlus | PORPHYROMONAS-GINGIVALIS | - |
dc.subject.keywordPlus | MATRIX METALLOPROTEINASES | - |
dc.subject.keywordPlus | FIMA GENOTYPES | - |
dc.subject.keywordPlus | SUBGINGIVAL PLAQUE | - |
dc.subject.keywordPlus | IMMUNE-RESPONSES | - |
dc.subject.keywordPlus | EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | POLYMORPHISMS | - |
dc.subject.keywordPlus | LIGAMENT | - |
dc.subject.keywordPlus | STRAINS | - |
dc.subject.keywordAuthor | Gingival fibroblast | - |
dc.subject.keywordAuthor | Periodontitis | - |
dc.subject.keywordAuthor | Porphyromonas gingivalis | - |
dc.subject.keywordAuthor | Inflammatory cytokines | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.