beta-Catenin activation contributes to the pathogenesis of adenomyosis through epithelial-mesenchymal transition
- Authors
- Oh, Seo Jin; Shin, Jung-Ho; Kim, Tae Hoon; Lee, Hee Sun; Yoo, Jung-Yoon; Ahn, Ji Yeon; Broaddus, Russell R.; Taketo, Makoto M.; Lydon, John P.; Leach, Richard E.; Lessey, Bruce A.; Fazleabas, Asgerally T.; Lim, Jeong Mook; Jeong, Jae-Wook
- Issue Date
- 10월-2013
- Publisher
- WILEY
- Keywords
- beta-catenin; adenomyosis; epithelial-mesenchymal transition; uterus
- Citation
- JOURNAL OF PATHOLOGY, v.231, no.2, pp.210 - 222
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF PATHOLOGY
- Volume
- 231
- Number
- 2
- Start Page
- 210
- End Page
- 222
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/101994
- DOI
- 10.1002/path.4224
- ISSN
- 0022-3417
- Abstract
- Adenomyosis is defined by the presence of endometrial glands and stroma within the myometrium. Despite its frequent occurrence, the precise aetiology and physiopathology of adenomyosis is still unknown. WNT/beta beta-catenin signalling molecules are important and should be tightly regulated for uterine function. To investigate the role of beta-catenin signalling in adenomyosis, the expression of beta-catenin was examined. Nuclear and cytoplasmic beta-catenin expression was significantly higher in epithelial cells of human adenomyosis compared to control endometrium. To determine whether constitutive activation of beta-catenin in the murine uterus leads to development of adenomyosis, mice that expressed a dominant stabilized beta-catenin in the uterus were used by crossing PR-Cre mice with Ctnnb1(f(ex3)/+) mice. Uteri of PRcre/+ Ctnnb1(f(ex3)/+) mice displayed an abnormal irregular structure and highly active proliferation in the myometrium, and subsequently developed adenomyosis. Interestingly, the expression of E-cadherin was repressed in epithelial cells of PRcre/+ Ctnnb1(f(ex3)/+) mice compared to control mice. Repression of E-cadherin is one of the hallmarks of epithelial-mesenchymal transition (EMT). The expression of SNAIL and ZEB1 was observed in some epithelial cells of the uterus in PRcre/+ Ctnnb1(f(ex3)/+) mice but not in control mice. Vimentin and COUP-TFII, mesenchymal cell markers, were expressed in some epithelial cells of PRcre/+ Ctnnb1(f(ex3)/+) mice. In human adenomyosis, the expression of E-cadherin was decreased in epithelial cells compared to control endometrium, while CD10, an endometrial stromal marker, was expressed in some epithelial cells of human adenomyosis. These results suggest that abnormal activation of beta-catenin contributes to adenomyosis development through the induction of EMT. Copyright (c) 2013 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
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