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Butyrate Production in Engineered Escherichia coli With Synthetic Scaffolds

Authors
Baek, Jang-MiMazumdar, SumanLee, Sang-WooJung, Moo-YoungLim, Jae-HyungSeo, Sang-WooJung, Gyoo-YeolOh, Min-Kyu
Issue Date
10월-2013
Publisher
WILEY-BLACKWELL
Keywords
Escherichia coli; butyrate; synthetic scaffold; metabolic engineering; heterologous pathway
Citation
BIOTECHNOLOGY AND BIOENGINEERING, v.110, no.10, pp.2790 - 2794
Indexed
SCIE
SCOPUS
Journal Title
BIOTECHNOLOGY AND BIOENGINEERING
Volume
110
Number
10
Start Page
2790
End Page
2794
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/102077
DOI
10.1002/bit.24925
ISSN
0006-3592
Abstract
Butyrate pathway was constructed in recombinant Escherichia coli using the genes from Clostridium acetobutylicum and Treponema denticola. However, the pathway constructed from exogenous enzymes did not efficiently convert carbon flux to butyrate. Three steps of the productivity enhancement were attempted in this study. First, pathway engineering to delete metabolic pathways to by-products successfully improved the butyrate production. Second, synthetic scaffold protein that spatially co-localizes enzymes was introduced to improve the efficiency of the heterologous pathway enzymes, resulting in threefold improvement in butyrate production. Finally, further optimizations of inducer concentrations and pH adjustment were tried. The final titer of butyrate was 4.3 and 7.2g/L under batch and fed-batch cultivation, respectively. This study demonstrated the importance of synthetic scaffold protein as a useful tool for optimization of heterologous butyrate pathway in E. coli. Biotechnol. Bioeng. 2013;110: 2790-2794. (c) 2013 Wiley Periodicals, Inc.
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