Specific detection of inflamed cells using TLR1 antibody and its secondary antibody-conjugated nano-beads
DC Field | Value | Language |
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dc.contributor.author | Yeo, Ki Baek | - |
dc.contributor.author | Ha, Unhwan | - |
dc.contributor.author | Jung, Yong Woo | - |
dc.contributor.author | Kang, Taek Jin | - |
dc.contributor.author | Pack, Seung Pil | - |
dc.date.accessioned | 2021-09-05T21:49:19Z | - |
dc.date.available | 2021-09-05T21:49:19Z | - |
dc.date.created | 2021-06-15 | - |
dc.date.issued | 2013-09-10 | - |
dc.identifier.issn | 0141-0229 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/102172 | - |
dc.description.abstract | Chronic inflammation can lead to several diseases, thus analysis of the inflammation state of live cells may have important clinical applications. However, there is not currently well-established method for specific detection of inflamed cells via live cell imaging. In this study, we developed an effective antibody (Ab)-based cell imaging method for the detection of inflamed cells using Ab-conjugated nano-beads. Several receptors were tested as potential biomarkers for cell inflammation, and corresponding fluorescence-labeled Abs and/or Ab-conjugated nano-beads were used to detect inflamed cells via fluorescence imaging. Interestingly, when we employed sequential use of TLR1 primary Ab and size-optimized nano-beads conjugated with secondary Ab, we were able to clearly discriminate inflamed cells from normal ones. The Ab-based cell-imaging method described herein provides an important basis for the development of high-throughput analysis of cell inflammation, potentially leading to the identification of factors involved in inflammation and anti-inflammatory drug candidates. (c) 2013 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.subject | TOLL-LIKE RECEPTORS | - |
dc.subject | PSEUDOMONAS-AERUGINOSA | - |
dc.subject | INFLAMMATION | - |
dc.title | Specific detection of inflamed cells using TLR1 antibody and its secondary antibody-conjugated nano-beads | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ha, Unhwan | - |
dc.contributor.affiliatedAuthor | Jung, Yong Woo | - |
dc.contributor.affiliatedAuthor | Pack, Seung Pil | - |
dc.identifier.doi | 10.1016/j.enzmictec.2013.06.001 | - |
dc.identifier.scopusid | 2-s2.0-84881542437 | - |
dc.identifier.wosid | 000324008200001 | - |
dc.identifier.bibliographicCitation | ENZYME AND MICROBIAL TECHNOLOGY, v.53, no.4, pp.223 - 228 | - |
dc.relation.isPartOf | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.citation.title | ENZYME AND MICROBIAL TECHNOLOGY | - |
dc.citation.volume | 53 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 223 | - |
dc.citation.endPage | 228 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | TOLL-LIKE RECEPTORS | - |
dc.subject.keywordPlus | PSEUDOMONAS-AERUGINOSA | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordAuthor | Inflammation | - |
dc.subject.keywordAuthor | TLR1 antibody | - |
dc.subject.keywordAuthor | Nano-sized beads | - |
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