Magnetic Separation of Malaria-Infected Red Blood Cells in Various Developmental Stages
DC Field | Value | Language |
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dc.contributor.author | Nam, Jeonghun | - |
dc.contributor.author | Huang, Hui | - |
dc.contributor.author | Lim, Hyunjung | - |
dc.contributor.author | Lim, Chaeseung | - |
dc.contributor.author | Shin, Sehyun | - |
dc.date.accessioned | 2021-09-05T22:59:39Z | - |
dc.date.available | 2021-09-05T22:59:39Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2013-08-06 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/102475 | - |
dc.description.abstract | Malaria is a serious disease that threatens the public health, especially in developing countries. Various methods have been developed to separate malaria-infected red blood cells (i-RBCs) from blood samples for clinical diagnosis and biological and epidemiological research. In this study, we propose a simple and label-free method for separating not only late-stage but also early-stage i-RBCs on the basis of their paramagnetic characteristics due to the malaria byproduct, hemozoin, by using a magnetic field gradient. A polydimethylsiloxane (PDMS) microfluidic channel was fabricated and integrated with a ferromagnetic wire fixed on a glass slide. To evaluate the performance of the microfluidic device containing the ferromagnetic wire, lateral displacement of NaNO2-treated RBCs, which also have paramagnetic characteristics, was observed at various flow rates. The results showed excellent agreement with theoretically predicted values. The same device was applied to separate i-RBCs. Late-stage i-RBCs (trophozoites and schizonts), which contain optically visible black dots, were separated with a recovery rate of approximately 98.3%. In addition, using an optimal flow rate, early-stage (ring-stage) i-RBCs, which had been difficult to separate because of their low paramagnetic characteristics, were successfully separated with a recovery rate of 73%. The present technique, using permanent magnets and ferromagnetic wire in a microchannel, can effectively separate i-RBCs in various developmental stages so that it could provide a potential tool for studying the invasion mechanism of the malarial parasite, as well as performing antimalarial drug assays. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | MODE MAGNETOPHORETIC MICROSEPARATOR | - |
dc.subject | FALCIPARUM | - |
dc.subject | GRADIENT | - |
dc.subject | ERYTHROCYTES | - |
dc.subject | DIAGNOSIS | - |
dc.subject | PARASITES | - |
dc.subject | TESTS | - |
dc.title | Magnetic Separation of Malaria-Infected Red Blood Cells in Various Developmental Stages | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lim, Chaeseung | - |
dc.contributor.affiliatedAuthor | Shin, Sehyun | - |
dc.identifier.doi | 10.1021/ac4012057 | - |
dc.identifier.scopusid | 2-s2.0-84881332774 | - |
dc.identifier.wosid | 000323014000048 | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.85, no.15, pp.7316 - 7323 | - |
dc.relation.isPartOf | ANALYTICAL CHEMISTRY | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 85 | - |
dc.citation.number | 15 | - |
dc.citation.startPage | 7316 | - |
dc.citation.endPage | 7323 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | MODE MAGNETOPHORETIC MICROSEPARATOR | - |
dc.subject.keywordPlus | FALCIPARUM | - |
dc.subject.keywordPlus | GRADIENT | - |
dc.subject.keywordPlus | ERYTHROCYTES | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | PARASITES | - |
dc.subject.keywordPlus | TESTS | - |
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