The inhibitory effect of a synthetic compound, (Z)-5-(2,4-dihydroxybenzylidene) thiazolidine-2,4-dione (MHY498), on nitric oxide-induced melanogenesis
- Authors
- Kim, So Hee; Choi, Yeon Ja; Moon, Kyoung Mi; Lee, Hye Jin; Woo, Youngwoo; Chung, Ki Wung; Jung, Yuri; Kim, Sora; Chun, Pusoon; Byun, Youngjoo; Ha, Young Mi; Moon, Hyung Ryong; Chung, Hae Young
- Issue Date
- 1-8월-2013
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- (Z)-5-(2,4-Dihydroxy benzylidene)thiazolidine-2,4-dione; Melanogenesis; Tyrosinase; Nitric oxide; Melanin; cGMP; MITF
- Citation
- BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, v.23, no.15, pp.4332 - 4335
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
- Volume
- 23
- Number
- 15
- Start Page
- 4332
- End Page
- 4335
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/102491
- DOI
- 10.1016/j.bmcl.2013.05.094
- ISSN
- 0960-894X
- Abstract
- Nitric oxide (NO) and the NO/PKG signaling pathway play crucial roles in ultraviolet (UV)-induced melanogenesis, which is known to be related to the induction of tyrosinase. In an attempt to find a novel anti-melanogenic agent, we synthesized (Z)-5-(2,4-dihydroxybenzylidene) thiazolidine-2,4-dione (MHY498). The purpose of this study was to investigate the effect of MHY498 on NO levels and on the NO-mediated signaling pathway using an in vitro model of melanogenesis. MHY498 inhibited 200 mu M sodium nitroprusside (SNP, a NO donor)-induced NO generation, dose-dependently and suppressed tyrosinase activity and melanin synthesis induced by SNP in B16F10 melanoma cells. To investigate the effect of MHY498 on NO-mediated signaling pathway, guanosine cyclic 3',5'-monophosphate (cGMP) activities were measured using a cGMP EIA Kit and western blotting was performed to determine the effects of MHY498 on the gene expressions of tyrosinase and microphthalmia-associated transcription factor (MITF). The increased activity of cGMP by SNP was reduced dose-dependently by pretreatment with MHY498. Furthermore, MHY498 suppressed the expressions of tyrosinase and MITF stimulated by SNP. This study shows that enhancement of tyrosinase gene expression via the cGMP pathway is a probable primary mechanism of NO-induced melanogenesis and that the NO-mediated signaling pathway with the expression of MITF enhances melanogenesis. In addition, MHY498 was found to scavenge NO and to suppress the activity of the NO-mediated signaling pathway, and thus, to subsequently down-regulate tyrosinase expression and melanogenesis. This study suggests that MHY498 is a promising anti-melanogenic agent that targets the NO-induced cGMP signaling pathway. (C) 2013 Elsevier Ltd. All rights reserved.
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