Extremely low-frequency electromagnetic fields induce neural differentiation in bone marrow derived mesenchymal stem cells
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyun-Jung | - |
dc.contributor.author | Jung, Jessica | - |
dc.contributor.author | Park, Jee-Hye | - |
dc.contributor.author | Kim, Jin-Hee | - |
dc.contributor.author | Ko, Kyung-Nam | - |
dc.contributor.author | Kim, Chan-Wha | - |
dc.date.accessioned | 2021-09-05T23:20:51Z | - |
dc.date.available | 2021-09-05T23:20:51Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2013-08 | - |
dc.identifier.issn | 1535-3702 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/102605 | - |
dc.description.abstract | Extremely low-frequency electromagnetic fields (ELF-EMF) affect numerous biological functions such as gene expression, cell fate determination and even cell differentiation. To investigate the correlation between ELF-EMF exposure and differentiation, bone marrow derived mesenchymal stem cells (BM-MSCs) were subjected to a 50-Hz electromagnetic field during in vitro expansion. The influence of ELF-EMF on BM-MSCs was analysed by a range of different analytical methods to understand its role in the enhancement of neural differentiation. ELF-EMF exposure significantly decreased the rate of proliferation, which in turn caused an increase in neuronal differentiation. The ELF-EMF-treated cells showed increased levels of neuronal differentiation marker (MAP2), while early neuronal marker (Nestin) was down-regulated. In addition, eight differentially expressed proteins were detected in two-dimensional electrophoresis maps, and were identified using ESI-Q-TOF LC/MS/MS. Among them, ferritin light chain, thioredoxin-dependent peroxide reductase, and tubulin beta-6 chain were up-regulated in the ELF-EMF-stimulated group. Ferritin and thioredoxin-dependent peroxide reductase are involved in a wide variety of functions, including Ca2+ regulation, which is a critical component of neurodegeneration. We also observed that the intracellular Ca2+ content was significantly elevated after ELF-EMF exposure, which strengthens the modulatory role of ferritin and thioredoxin-dependent peroxide reductase, during differentiation. Notably, western blot analysis indicated significantly increased expression of the ferritin light chain in the ELF-EMF-stimulated group (0.60 vs. 1.08; P < 0.01). These proteins may help understand the effect of ELF-EMF stimulation on BM-MSCs during neural differentiation and its potential use as a clinically therapeutic option for treating neurodegenerative diseases. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC MEDICINE PRESS LTD | - |
dc.subject | DEPENDENT PEROXIDE REDUCTASE | - |
dc.subject | TUBULIN ISOTYPES | - |
dc.subject | PROTEOMIC ANALYSIS | - |
dc.subject | MAGNETIC-FIELDS | - |
dc.subject | MESSENGER-RNAS | - |
dc.subject | RAT-BRAIN | - |
dc.subject | EXPRESSION | - |
dc.subject | FERRITIN | - |
dc.subject | PROTEIN | - |
dc.subject | IRON | - |
dc.title | Extremely low-frequency electromagnetic fields induce neural differentiation in bone marrow derived mesenchymal stem cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Chan-Wha | - |
dc.identifier.doi | 10.1177/1535370213497173 | - |
dc.identifier.scopusid | 2-s2.0-84884524110 | - |
dc.identifier.wosid | 000323510400010 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL BIOLOGY AND MEDICINE, v.238, no.8, pp.923 - 931 | - |
dc.relation.isPartOf | EXPERIMENTAL BIOLOGY AND MEDICINE | - |
dc.citation.title | EXPERIMENTAL BIOLOGY AND MEDICINE | - |
dc.citation.volume | 238 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 923 | - |
dc.citation.endPage | 931 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | DEPENDENT PEROXIDE REDUCTASE | - |
dc.subject.keywordPlus | TUBULIN ISOTYPES | - |
dc.subject.keywordPlus | PROTEOMIC ANALYSIS | - |
dc.subject.keywordPlus | MAGNETIC-FIELDS | - |
dc.subject.keywordPlus | MESSENGER-RNAS | - |
dc.subject.keywordPlus | RAT-BRAIN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | FERRITIN | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | IRON | - |
dc.subject.keywordAuthor | Extremely low-frequency electromagnetic fields | - |
dc.subject.keywordAuthor | BM-MSCs | - |
dc.subject.keywordAuthor | neural differentiation | - |
dc.subject.keywordAuthor | ferritin | - |
dc.subject.keywordAuthor | Ca2+ regulation | - |
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