Engineered Enterobacter aerogenes for efficient utilization of sugarcane molasses in 2,3-butanediol production
- Authors
- Jung, Moo-Young; Park, Bu-Soo; Lee, Jinwon; Oh, Min-Kyu
- Issue Date
- 7월-2013
- Publisher
- ELSEVIER SCI LTD
- Keywords
- 2,3-Butanediol; Sugarcane molasses; Enterobacter aerogenes; Sucrose regulator; Fermentation
- Citation
- BIORESOURCE TECHNOLOGY, v.139, pp.21 - 27
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 139
- Start Page
- 21
- End Page
- 27
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/102852
- DOI
- 10.1016/j.biortech.2013.04.003
- ISSN
- 0960-8524
- Abstract
- Sugarcane molasses is considered to be a good carbon source for biorefinery due to its high sugar content and low price. Sucrose occupies more than half of the sugar in the molasses. Enterobacter aerogenes is a good host strain for 2,3-butanediol production, but its utilization of sucrose is not very efficient. To improve sucrose utilization in E. aerogenes, a sucrose regulator (ScrR) was disrupted from the genomic DNA. The deletion mutation increased the sucrose consumption rate significantly when sucrose or sugarcane molasses was used as a carbon source. The 2,3-butanediol production from sugarcane molasses by the mutant was enhanced by 60% in batch fermentation compared to that by the wild type strain. In fed-batch fermentation, 98.69 g/L of 2,3-butanediol production was achieved at 36 h. (C) 2013 Elsevier Ltd. All rights reserved.
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