Biodiesel production by enzymatic process using Jatropha oil and waste soybean oil
- Authors
- Lee, Ja Hyun; Kim, Sung Bong; Yoo, Hah Young; Suh, Young Joon; Kang, Gyung Bo; Jang, Woo In; Kang, Jongwon; Park, Chulhwan; Kim, Seung Wook
- Issue Date
- 7월-2013
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
- Keywords
- biodiesel; continuous process; lipase; response surface methodology; optimization
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.18, no.4, pp.703 - 708
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- Volume
- 18
- Number
- 4
- Start Page
- 703
- End Page
- 708
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/102930
- DOI
- 10.1007/s12257-012-0805-8
- ISSN
- 1226-8372
- Abstract
- In this study, non-edible Jatropha oil and postcooking waste soybean oil were utilized for enzymatic biodiesel production. The process was optimized by using a statistical method. In addition, a novel continuous process using co-immobilized Rhizopus oryzae and Candida rugosa lipases was developed. The optimum conditions for the batch process were determined to be a reaction temperature of 45oC, an agitation speed of 250 rpm, 10 wt% of water, and 20% of immobilized lipases. A conversion of about 98% at 4 h could be achieved for biodiesel production using Jatropha oil, while a conversion of about 97% at 4 h was achieved from waste soybean oil. A packed bed reactor charged with co-immobilized lipases was employed for continuous biodiesel production from Jatropha and waste soybean oil. The reactor consisted of a jacketed glass column (ID 25 mm x 130 mm), in which a temperature of 45A degrees C was maintained by water circulation. A maximum conversion of about 80% in 24 h at a flow rate of 0.8 mL/ min was achieved with the continuous process, whereas in the two-stage continuous process, a conversion of about 90% in 72 h was attained at a flow rate of 0.1 mL/min.
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