Kdx1 regulates RCK1 gene expression by interacting with Rlm1 in Saccharomyces cerevisiae
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chang, Miwha | - |
dc.contributor.author | Kang, Hyun-Jun | - |
dc.contributor.author | Baek, In-Joon | - |
dc.contributor.author | Kang, Chang-Min | - |
dc.contributor.author | Park, Yong-Sung | - |
dc.contributor.author | Yun, Cheol-Won | - |
dc.date.accessioned | 2021-09-06T00:42:21Z | - |
dc.date.available | 2021-09-06T00:42:21Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2013-06-07 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/102982 | - |
dc.description.abstract | Kdx1 is known as a stress-responsive protein. To better understand the function of Kdx1, we performed microarray analysis in KDX1 overexpressing cells and found that the overexpression of KDX1 dramatically induced the expression of RCK1, a stress-responsive gene. This result was confirmed by northern blot analysis. Furthermore, the overexpression of RCK1 partially rescued the growth defect caused by zymolyase stress. The expression of RCK1 was regulated independently by Slt2 and Hog1, and Kdx1 failed to induce the expression of RCK1 in a HOG1 deletion strain. The transcriptional factors Smp1, Sko1, Msn2, Msn4, and Hot1, which are regulated by Hog1, did not affect RCK1 expression, but Rlm1 did. Furthermore, the mutation of certain phosphorylation sites in RLM1 inhibited the induction of RCK1 expression by Kdx1. We found a conserved Rlm1 binding site in the 5' untranslated region (UTR) of RCK1, and the mutation of these Rlm1 binding sites also inhibited the induction of RCK1 expression by Kdx1. Finally, we showed that Kdx1 physically interacts with RIm1 and that this interaction affects the ability of Rlm1 to bind to the RCK1 5' UTR. Taken together, these data suggest that Kdx1 interacts with Rlm1 to activate RCK1 gene expression in response to stress in Saccharomyces cerevisiae. (C) 2013 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | SIGNAL-TRANSDUCTION PATHWAY | - |
dc.subject | MAP KINASE PATHWAY | - |
dc.subject | PROTEIN-KINASE | - |
dc.subject | TRANSCRIPTION FACTOR | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | HOG PATHWAY | - |
dc.subject | YEAST | - |
dc.subject | SLT2 | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | RESISTANCE | - |
dc.title | Kdx1 regulates RCK1 gene expression by interacting with Rlm1 in Saccharomyces cerevisiae | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yun, Cheol-Won | - |
dc.identifier.doi | 10.1016/j.bbrc.2013.04.083 | - |
dc.identifier.scopusid | 2-s2.0-84879003855 | - |
dc.identifier.wosid | 000320904900005 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.435, no.3, pp.350 - 355 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 435 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 350 | - |
dc.citation.endPage | 355 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION PATHWAY | - |
dc.subject.keywordPlus | MAP KINASE PATHWAY | - |
dc.subject.keywordPlus | PROTEIN-KINASE | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTOR | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | HOG PATHWAY | - |
dc.subject.keywordPlus | YEAST | - |
dc.subject.keywordPlus | SLT2 | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordAuthor | Saccharomyces cerevisiae | - |
dc.subject.keywordAuthor | Rck1 | - |
dc.subject.keywordAuthor | Kdx1 | - |
dc.subject.keywordAuthor | Hog1 | - |
dc.subject.keywordAuthor | Cell wall stress | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.