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Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production

Authors
Lee, Jong HoKim, Sung BongYoo, Hah YoungLee, Ja HyunHan, Sung OkPark, ChulhwanKim, Seung Wook
Issue Date
6월-2013
Publisher
KOREAN INSTITUTE CHEMICAL ENGINEERS
Keywords
Biodiesel; Enzymatic Process; Co-immobilization; Lipase
Citation
KOREAN JOURNAL OF CHEMICAL ENGINEERING, v.30, no.6, pp.1335 - 1338
Indexed
SCIE
SCOPUS
KCI
Journal Title
KOREAN JOURNAL OF CHEMICAL ENGINEERING
Volume
30
Number
6
Start Page
1335
End Page
1338
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/103027
DOI
10.1007/s11814-013-0058-z
ISSN
0256-1115
Abstract
Candida rugosa lipase and Ryzopus oryzae lipase were simultaneously immobilized on silica gel following enzyme pretreatment. The factors affecting the co-immobilization process, such as reaction time and enzyme ratio, were investigated. Biodiesel was then produced by using the co-immobilized enzyme matrix. A batch system was employed with stepwise methanol feeding, and the continuous process involved a packed-bed reactor. Under optimal immobilization conditions, the activity was approximately 16,000 U/g center dot matrix. When co-immobilized enzyme was used with optimized stepwise methanol feeding, conversion of biodiesel reached about 99% at 3 h and was maintained at a level of over 90% for about 30 reuses.
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Graduate School > Department of Biotechnology > 1. Journal Articles
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