A three-dimensional microfluidic tumor cell migration assay to screen the effect of anti-migratory drugs and interstitial flow
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kalchman, Johann | - |
dc.contributor.author | Fujioka, Shingo | - |
dc.contributor.author | Chung, Seok | - |
dc.contributor.author | Kikkawa, Yamato | - |
dc.contributor.author | Mitaka, Toshihiro | - |
dc.contributor.author | Kamm, Roger D. | - |
dc.contributor.author | Tanishita, Kazuo | - |
dc.contributor.author | Sudo, Ryo | - |
dc.date.accessioned | 2021-09-06T00:58:49Z | - |
dc.date.available | 2021-09-06T00:58:49Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2013-06 | - |
dc.identifier.issn | 1613-4982 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/103056 | - |
dc.description.abstract | Most anti-cancer drug screening assays are currently performed in two dimensions, on flat, rigid surfaces. However, there are increasing indications that three-dimensional (3D) platforms provide a more realistic setting to investigate accurate morphology, growth, and sensitivity of tumor cells to chemical factors. Moreover, interstitial flow plays a pivotal role in tumor growth. Here, we present a microfluidic 3D platform to investigate behaviors of tumor cells in flow conditions with anti-migratory compounds. Our results show that interstitial flow and its direction have significant impact on migration and growth of hepatocellular carcinoma cell lines such as HepG2 and HLE. In particular, HepG2/HLE cells tend to migrate against interstitial flow, and their growth increases in interstitial flow conditions regardless of the flow direction. Furthermore, this migratory activity of HepG2 cells is enhanced when they are co-cultured with human umbilical vein endothelial cells. We also found that migration activity of HepG2 cells attenuates under hypoxic conditions. In addition, the effect of Artemisinin, an anti-migratory compound, on HepG2 cells was quantitatively analyzed. The microfluidic 3D platform described here is useful to investigate more accurately the effect of anti-migratory drugs on tumor cells and the critical influence of interstitial flow than 2D culture models. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.subject | IN-VITRO | - |
dc.subject | CANCER | - |
dc.subject | INVASION | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | ANGIOGENESIS | - |
dc.subject | EXPRESSION | - |
dc.subject | RESISTANCE | - |
dc.subject | APOPTOSIS | - |
dc.subject | PRESSURE | - |
dc.subject | HYPOXIA | - |
dc.title | A three-dimensional microfluidic tumor cell migration assay to screen the effect of anti-migratory drugs and interstitial flow | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chung, Seok | - |
dc.identifier.doi | 10.1007/s10404-012-1104-6 | - |
dc.identifier.scopusid | 2-s2.0-84879694397 | - |
dc.identifier.wosid | 000320857900006 | - |
dc.identifier.bibliographicCitation | MICROFLUIDICS AND NANOFLUIDICS, v.14, no.6, pp.969 - 981 | - |
dc.relation.isPartOf | MICROFLUIDICS AND NANOFLUIDICS | - |
dc.citation.title | MICROFLUIDICS AND NANOFLUIDICS | - |
dc.citation.volume | 14 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 969 | - |
dc.citation.endPage | 981 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Physics, Fluids & Plasmas | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | INVASION | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | PRESSURE | - |
dc.subject.keywordPlus | HYPOXIA | - |
dc.subject.keywordAuthor | 3D cell migration | - |
dc.subject.keywordAuthor | Microfluidics | - |
dc.subject.keywordAuthor | Anti-migratory drugs | - |
dc.subject.keywordAuthor | Interstitial flow | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.