Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Indole toxicity involves the inhibition of adenosine triphosphate production and protein folding in Pseudomonas putida

Authors
Kim, JisunHong, HyerimHeo, AramPark, Woojun
Issue Date
6월-2013
Publisher
WILEY-BLACKWELL
Keywords
indole; microarray; NADH; NAD plus ratio; ATP; protein folding; Pseudomonas
Citation
FEMS MICROBIOLOGY LETTERS, v.343, no.1, pp.89 - 99
Indexed
SCIE
SCOPUS
Journal Title
FEMS MICROBIOLOGY LETTERS
Volume
343
Number
1
Start Page
89
End Page
99
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/103087
DOI
10.1111/1574-6968.12135
ISSN
0378-1097
Abstract
High concentrations of indole are known to be toxic to cells due to perturbations in membrane potential. Here, we report for the first time a transcriptome analysis of a soil model bacterium, Pseudomonas putida KT2440, under indole treatment. We demonstrated that 47 genes are differentially expressed, including 11 genes involved in the tricarboxylic acid cycle (TCA cycle) and 12 genes involved in chaperone and protease functions (hslV, hslU, htpG, grpE, dnaK, ibpA, groEL, groES, clpB, lon-1, lon-2, and hflk). Mutant analysis supported the observation that protease genes including hslU are essential for the indole resistance of Pseudomonas strains. Subsequent biochemical analyses have shown that indole increases the NADH/NAD+ ratio and decreases the adenosine triphosphate (ATP) concentration inside cells, due to membrane perturbation and higher expression of TCA cycle genes in the presence of indole. This energy reduction leads to a reduction in cell size and an enhancement of biofilm formation in P.putida. The observed upregulation in many chaperones and proteases led us to speculate that protein folding might be inhibited by indole treatment. Interestingly, our in vitro protein-refolding assay using malate dehydrogenase with purified GroEL/GroES demonstrated that indole interferes with protein folding. Taken together, our data provide new evidence that indole causes toxicity to P.putida by inhibiting cellular energy production and protein folding.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Life Sciences and Biotechnology > Division of Environmental Science and Ecological Engineering > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Park, Woo jun photo

Park, Woo jun
생명과학대학 (환경생태공학부)
Read more

Altmetrics

Total Views & Downloads

BROWSE