1,25-dihydroxyvitamin D-3 inhibits directly human osteoclastogenesis by down-regulation of the c-Fms and RANK expression
DC Field | Value | Language |
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dc.contributor.author | Kim, Tae-Hwan | - |
dc.contributor.author | Lee, Bitnara | - |
dc.contributor.author | Kwon, Eunji | - |
dc.contributor.author | Choi, Choong Hyeok | - |
dc.contributor.author | Sung, Il-Hoon | - |
dc.contributor.author | Kim, Yongjin | - |
dc.contributor.author | Sohn, Jeongwon | - |
dc.contributor.author | Ji, Jong Dae | - |
dc.date.accessioned | 2021-09-06T01:55:22Z | - |
dc.date.available | 2021-09-06T01:55:22Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2013-05 | - |
dc.identifier.issn | 1297-319X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/103316 | - |
dc.description.abstract | Objective: 1,25-dihydroxyvitamin D-3 (1,25(OH)(2)D-3) is a key molecule to maintain calcium homeostasis and bone metabolism. It was recently reported that 1,25(OH)(2)D-3 directly inhibited osteoclast differentiation in mouse bone marrow cells and human bone marrow-derived colony-forming unit granulocyte macrophage (CFU-GM) cells. However, the direct effects of 1,25(OH)(2)D-3 and its affecting mechanisms on the osteoclast differentiation of human osteoclast precursors remain largely unknown. In this study, we examined the direct effects of 1,25(OH)(2)D-3 on the osteoclastogenesis of human peripheral blood (PB) osteoclast precursors. Methods: In vitro osteoclastogenesis assays were performed using osteoclast precursors from normal PB. The gene expressions were analyzed using real-time PCR. The cell surface proteins, including c-Fms and RANK, were measured by flow cytometry. Results: 1,25(OH)(2)D-3 strongly inhibited osteoclast differentiation and it suppressed the expression of RANK in the human PB osteoclast precursors. One mechanism of RANK inhibition by 1,25(OH)(2)D-3 is down-regulation of the M-CSF receptor c-Fms, which is required for the expression of RANK. In contrast to the previous reports on mouse osteoclast precursors, 1,25(OH)(2)D-3 did not affect the expression of c-Fos. Parallel to the inhibition of osteoclastogenesis, 1,25(OH)(2)D-3 increased the expression and phosphorylation of CCAAT enhancer-binding protein beta (C/EBP beta), which is a recently discovered inhibitor of osteoclastogenesis. Conclusions: Our results show that 1,25(OH)(2)D-3 inhibits human osteoclastogenesis by decreasing the RANK+ osteoclast precursors, and we suggest that 1,25(OH)(2)D-3 may be a powerful therapeutic agent for treating inflammation-induced bone disease that shows excessive osteoclast activation. (C) 2012 Societe francaise de rhumatologie. Published by Elsevier Masson SAS. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER | - |
dc.subject | VITAMIN-D | - |
dc.subject | BONE-MARROW | - |
dc.subject | RHEUMATOID-ARTHRITIS | - |
dc.subject | RAT MODEL | - |
dc.subject | IN-VIVO | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | CELLS | - |
dc.subject | OSTEOPOROSIS | - |
dc.subject | PRECURSORS | - |
dc.subject | RECEPTOR | - |
dc.title | 1,25-dihydroxyvitamin D-3 inhibits directly human osteoclastogenesis by down-regulation of the c-Fms and RANK expression | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sohn, Jeongwon | - |
dc.contributor.affiliatedAuthor | Ji, Jong Dae | - |
dc.identifier.doi | 10.1016/j.jbspin.2012.09.011 | - |
dc.identifier.scopusid | 2-s2.0-84878352012 | - |
dc.identifier.wosid | 000320602500016 | - |
dc.identifier.bibliographicCitation | JOINT BONE SPINE, v.80, no.3, pp.307 - 314 | - |
dc.relation.isPartOf | JOINT BONE SPINE | - |
dc.citation.title | JOINT BONE SPINE | - |
dc.citation.volume | 80 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 307 | - |
dc.citation.endPage | 314 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Rheumatology | - |
dc.relation.journalWebOfScienceCategory | Rheumatology | - |
dc.subject.keywordPlus | VITAMIN-D | - |
dc.subject.keywordPlus | BONE-MARROW | - |
dc.subject.keywordPlus | RHEUMATOID-ARTHRITIS | - |
dc.subject.keywordPlus | RAT MODEL | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | OSTEOPOROSIS | - |
dc.subject.keywordPlus | PRECURSORS | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordAuthor | 1,25-dihydroxyvitamin D-3 | - |
dc.subject.keywordAuthor | Osteoclast | - |
dc.subject.keywordAuthor | C-Fms | - |
dc.subject.keywordAuthor | RANK | - |
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