A simultaneous analysis method for Caihu-Dayuan-Yin using reversed-phase high-performance liquid chromatography coupled with pulsed amperometric detection

Abstract

A simultaneous analysis method was developed for Caihu-Dayuan-Yin decoction containing Bupleuri Radix, Glycyrrhizae Radix, and Ponciri fructus by reversed-phase (RP) HPLC coupled with pulsed amperometric detection (PAD). The analytes were separated in a RP column with 0.01 % phosphoric acid-acetonitrile gradient elution. The limits of detection (S/N = 3) and of quantification (S/N = 10) ranged 0.1-3.0, and 0.3-10 ng, respectively. The intra- and interday precisions (RSDs) were a parts per thousand currency sign15.15 % and the average recoveries were 94.6-98.3 %. Our RP-HPLC-PAD method showed 1.7-100 times higher sensitivity than the RP-HPLC-UV method.