High temperature and low acid pretreatment and agarase treatment of agarose for the production of sugar and ethanol from red seaweed biomass
- Authors
- Kim, Hee Taek; Yun, Eun Ju; Wang, Damao; Chung, Jae Hyuk; Choi, In-Geol; Kim, Kyoung Heon
- Issue Date
- 5월-2013
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Acid pretreatment; Agarase; Ethanol; Red algae
- Citation
- BIORESOURCE TECHNOLOGY, v.136, pp.582 - 587
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 136
- Start Page
- 582
- End Page
- 587
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/103340
- DOI
- 10.1016/j.biortech.2013.03.038
- ISSN
- 0960-8524
- Abstract
- To obtain fermentable sugar from agarose, pretreatment of agarose by using acetic acid was conducted for short durations (10-30 min) at low acid concentrations (1-5% (w/v)) and high temperatures (110-130 degrees C). On testing the pretreated agarose by using an endo-beta-agarase I (DagA), an exo-beta-agarase II (Aga50D), and neoagarobiose hydrolase (NABH), we observed that the addition of the endo-type agarase did not increase the sugar yield. Use of the crude enzyme of Vibrio sp. EJY3 in combination with Aga50D and NABH including acetic acid pretreatment resulted in a 1.3-fold increase in the final reducing sugar yield (62.8% of theoretical maximum based on galactose and 3,6-anhydrogalactose in the initial agarose), compared to those obtained using Aga50D and NABH only after acetic acid pretreatment. The simultaneous saccharification and fermentation of pretreated agarose yielded ethanol of 37.1% theoretical maximum yield from galactose contained in the pretreated agarose. (C) 2013 Elsevier Ltd. All rights reserved.
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