Celastrol suppresses breast cancer MCF-7 cell viability via the AMP-activated protein kinase (AMPK)-induced p53 polo like kinase 2 (PLK-2) pathway
DC Field | Value | Language |
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dc.contributor.author | Kim, Ji Hae | - |
dc.contributor.author | Lee, Jung Ok | - |
dc.contributor.author | Lee, Soo Kyung | - |
dc.contributor.author | Kim, Nami | - |
dc.contributor.author | You, Ga Young | - |
dc.contributor.author | Moon, Ji Wook | - |
dc.contributor.author | Sha, Jie | - |
dc.contributor.author | Kim, Su Jin | - |
dc.contributor.author | Park, Sun Hwa | - |
dc.contributor.author | Kim, Hyeon Soo | - |
dc.date.accessioned | 2021-09-06T03:10:19Z | - |
dc.date.available | 2021-09-06T03:10:19Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2013-04 | - |
dc.identifier.issn | 0898-6568 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/103662 | - |
dc.description.abstract | Celastrol, an anti-oxidant flavonoid that is widely distributed in the plant kingdom, has been suggested to have chemopreventive effects on cancer cells: however, the mechanism of this process is not completely understood. In this study, we found that celastrol suppressed the viability of breast cancer MCF-7 cells in an AMP-activated protein kinase (AMPK)-dependent fashion. Celastrol also induced an increase in reactive oxygen species (ROS) levels, leading to AMPK phosphorylation. Protein kinase C (PKC) zeta was also shown to play a role in celastrol-induced ROS generation. In addition, celastrol increased phosphorylation of the pro-apoptotic effector, p53. Inhibition of AMPK blocked celastrol-mediated p53 phosphorylation. Moreover, celastrol increased the expression of tumor suppressor polo like kinase-2 (PLK-2) in a p53-dependent manner. Neither celastrol-induced PLK-2 induction nor celastrol-mediated apoptosis inducing factor poly(ADP-ribose) polymerase-2 (PARP-2) induction was observed in p53 knock-out cells. Furthermore, add-back of PLK-2 resulted in an increase in both celastrol-mediated PARP-2 induction and celastrol-induced apoptotic index sub G1 population. Together, these results suggest that celastrol may have antitumor effects on MCF-7 cells via AMPK-induced p53 and PLK-2 pathways. (C) 2013 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | SYSTEMIC-LUPUS-ERYTHEMATOSUS | - |
dc.subject | ENERGY STATUS | - |
dc.subject | KEY SENSOR | - |
dc.subject | NUDE-MICE | - |
dc.subject | APOPTOSIS | - |
dc.subject | GROWTH | - |
dc.subject | HSP90 | - |
dc.subject | TRITERPENE | - |
dc.subject | TRIPTERINE | - |
dc.title | Celastrol suppresses breast cancer MCF-7 cell viability via the AMP-activated protein kinase (AMPK)-induced p53 polo like kinase 2 (PLK-2) pathway | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Sun Hwa | - |
dc.contributor.affiliatedAuthor | Kim, Hyeon Soo | - |
dc.identifier.doi | 10.1016/j.cellsig.2012.12.005 | - |
dc.identifier.scopusid | 2-s2.0-84873313731 | - |
dc.identifier.wosid | 000317161700011 | - |
dc.identifier.bibliographicCitation | CELLULAR SIGNALLING, v.25, no.4, pp.805 - 813 | - |
dc.relation.isPartOf | CELLULAR SIGNALLING | - |
dc.citation.title | CELLULAR SIGNALLING | - |
dc.citation.volume | 25 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 805 | - |
dc.citation.endPage | 813 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | SYSTEMIC-LUPUS-ERYTHEMATOSUS | - |
dc.subject.keywordPlus | ENERGY STATUS | - |
dc.subject.keywordPlus | KEY SENSOR | - |
dc.subject.keywordPlus | NUDE-MICE | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | HSP90 | - |
dc.subject.keywordPlus | TRITERPENE | - |
dc.subject.keywordPlus | TRIPTERINE | - |
dc.subject.keywordAuthor | AMPK | - |
dc.subject.keywordAuthor | Breast tumor | - |
dc.subject.keywordAuthor | Celastrol | - |
dc.subject.keywordAuthor | p53 | - |
dc.subject.keywordAuthor | PKCzeta | - |
dc.subject.keywordAuthor | PLK-2 | - |
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