Recombinant tagging system using ribosomal frameshifting to monitor protein expression
DC Field | Value | Language |
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dc.contributor.author | Han, Se Jong | - |
dc.contributor.author | Cho, Sayeon | - |
dc.contributor.author | Lowehhaupt, Ky | - |
dc.contributor.author | Park, So-Young | - |
dc.contributor.author | Sim, Sang Jun | - |
dc.contributor.author | Kim, Yang-Gyun | - |
dc.date.accessioned | 2021-09-06T04:14:06Z | - |
dc.date.available | 2021-09-06T04:14:06Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2013-03 | - |
dc.identifier.issn | 0006-3592 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/103924 | - |
dc.description.abstract | For rapid and accurate quantitation of recombinant proteins during expression and after purification, we introduce a new tagging strategy that expresses both target proteins and limitedly tagged target proteins together in a single cell at a constant ratio by utilizing cis-elements of programmed -1 ribosomal frameshifting (-1RFS) as an embedded device. -1RFS is an alternative reading mechanism that effectively controls protein expression by many viruses. When a target gene is fused to the enhanced green fluorescent protein (EGFP) gene with a -1RFS element implanted between them, the unfused target and the target-GFP fusion proteins are expressed at a fixed ratio. The expression ratio between these two protein products is adjustable simply by changing -1RFS signals. This limited-tagging system would be valuable for the real-time monitoring of protein expression when optimizing expression condition for a new protein, and in monitoring large-scale bioprocesses without a large metabolic burden on host cells. Furthermore, this strategy allows for the direct measurement of the quantity of a protein on a chip surface and easy application to proteomewide study of gene products. Biotechnol. Bioeng. 2013; 110: 898904. (c) 2012 Wiley Periodicals, Inc. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.subject | GREEN FLUORESCENT PROTEIN | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | RNA PSEUDOKNOT | - |
dc.subject | IN-VIVO | - |
dc.subject | CELL | - |
dc.subject | CORONAVIRUS | - |
dc.subject | CULTIVATION | - |
dc.subject | PROMOTER | - |
dc.subject | GROWTH | - |
dc.subject | CHIP | - |
dc.title | Recombinant tagging system using ribosomal frameshifting to monitor protein expression | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sim, Sang Jun | - |
dc.identifier.doi | 10.1002/bit.24740 | - |
dc.identifier.scopusid | 2-s2.0-84872660629 | - |
dc.identifier.wosid | 000313806400023 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOENGINEERING, v.110, no.3, pp.898 - 904 | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.citation.title | BIOTECHNOLOGY AND BIOENGINEERING | - |
dc.citation.volume | 110 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 898 | - |
dc.citation.endPage | 904 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.subject.keywordPlus | GREEN FLUORESCENT PROTEIN | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | RNA PSEUDOKNOT | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | CELL | - |
dc.subject.keywordPlus | CORONAVIRUS | - |
dc.subject.keywordPlus | CULTIVATION | - |
dc.subject.keywordPlus | PROMOTER | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | CHIP | - |
dc.subject.keywordAuthor | ribosomal frameshifting | - |
dc.subject.keywordAuthor | protein expression | - |
dc.subject.keywordAuthor | tagging system | - |
dc.subject.keywordAuthor | fluorescence | - |
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