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Lipase from Penicillium camembertii KCCM 11268: Optimization of solid state fermentation and application to biodiesel production

Authors
Malilas, WarapornKang, Seong WooKim, Sung BongYoo, Hah YoungChulalaksananukul, WarawutKim, Seung Wook
Issue Date
2월-2013
Publisher
KOREAN INSTITUTE CHEMICAL ENGINEERS
Keywords
Penicillium camembertii KCCM 11268; Solid State Fermentation; Lipase Production; Statistical Experimental Design; Biodiesel Production; Immobilization
Citation
KOREAN JOURNAL OF CHEMICAL ENGINEERING, v.30, no.2, pp.405 - 412
Indexed
SCIE
SCOPUS
KCI
Journal Title
KOREAN JOURNAL OF CHEMICAL ENGINEERING
Volume
30
Number
2
Start Page
405
End Page
412
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/104011
DOI
10.1007/s11814-012-0132-y
ISSN
0256-1115
Abstract
Lipase was produced by Penicillium camembertii KCCM 11268 under solid state fermentation (SSF), and the production process was optimized by using statistical experimental designs. The initial moisture content, cultivation time, inoculum size and concentration of basal medium were considered as the factors of optimum conditions for SSF. P. Camembertii KCCM 11268 was cultivated in SSF using wheat bran as the substrate for lipase production. Under the optimized condition, lipase activity was reached around 7.8 U/ml after eight days fermentation. To partially purify the lipase, ammonium sulfate (80% saturation) was added to the crude lipase solution and concentrated using a diafiltration (VIVAFLOW 50). The concentrated lipase solution from P. Camembertii KCCM 11268 (PCL) was immobilized on silica gel by cross-linking method. Also, PCL was mixed with a commercial lipase solution from Candida rugosa (CRL), and this mixture was co-immobilized on silica gel. The immobilized and co-immobilized lipase activities were 1150.1 and 7924.8 U/g matrix, respectively. Palm oil and methanol were used as substrates and 1mmol of methanol was added every 1.5 h and 2 times during biodiesel production. The reaction was carried out at temperatures of 30, 40, 50, 60 and 70 A degrees C. The maximum biodiesel conversion by co-immobilized lipase was 99% after 5 h at 50 A degrees C.
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