Microarray analysis of Drosophila dicer-2 mutants reveals potential regulation of mitochondrial metabolism by endogenous siRNAs
DC Field | Value | Language |
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dc.contributor.author | Lim, Do-Hwan | - |
dc.contributor.author | Lee, Langho | - |
dc.contributor.author | Oh, Chun-Taek | - |
dc.contributor.author | Kim, Nam-Hoon | - |
dc.contributor.author | Hwang, Seungwoo | - |
dc.contributor.author | Han, Sung-Jun | - |
dc.contributor.author | Lee, Young Sik | - |
dc.date.accessioned | 2021-09-06T04:36:56Z | - |
dc.date.available | 2021-09-06T04:36:56Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2013-02 | - |
dc.identifier.issn | 0730-2312 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/104022 | - |
dc.description.abstract | RNA interference is a eukaryotic regulatory mechanism by which small non-coding RNAs typically mediate specific silencing of their cognate genes. In Drosophila, the RNase III enzyme Dicer-2 (Dcr-2) is essential for biogenesis of endogenous small interfering RNAs (endo-siRNAs), which have been implicated in regulation of endogenous protein-coding genes. Although much is known about microRNA-based regulatory networks, the biological functions of endo-siRNAs in animals remain poorly understood. We performed gene expression profiling on Drosophila dcr-2 null mutant pupae to investigate transcriptional effects caused by a severe defect in endo-siRNA production, and found 306 up-regulated and 357 down-regulated genes with at least a twofold change in expression compared with the wild type. Most of these up-regulated and down-regulated genes were associated with energy metabolism and development, respectively. Importantly, mRNA sequences of 39% of the up-regulated genes were perfectly complementary to the sequences of previously reported endo-siRNAs, suggesting they may be direct targets of endo-siRNAs. We confirmed up-regulation of five selected genes matching endo-siRNAs and concomitant down-regulation of the corresponding endo-siRNAs in dcr-2 mutant pupae. Most of the potential endo-siRNA target genes were associated with energy metabolism, including the citric acid cycle and oxidative phosphorylation in mitochondria, implying that these are major metabolic processes directly affected by endo-siRNAs in Drosophila. Consistent with this finding, dcr-2 null mutant pupae had lower ATP content compared with controls, indicating that mitochondrial energy production is impaired in these mutants. Our data support a potential role for the endo-siRNA pathway in energy homeostasis through regulation of mitochondrial metabolism. J. Cell. Biochem. 114: 418427, 2013. (c) 2012 Wiley Periodicals, Inc. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | LIFE-SPAN | - |
dc.subject | INTERFERING RNAS | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | SOMATIC-CELLS | - |
dc.subject | PATHWAY | - |
dc.subject | LONGEVITY | - |
dc.subject | COMPLEX | - |
dc.subject | MICE | - |
dc.subject | EXPRESSION | - |
dc.subject | DISEASES | - |
dc.title | Microarray analysis of Drosophila dicer-2 mutants reveals potential regulation of mitochondrial metabolism by endogenous siRNAs | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Young Sik | - |
dc.identifier.doi | 10.1002/jcb.24379 | - |
dc.identifier.scopusid | 2-s2.0-84872708386 | - |
dc.identifier.wosid | 000312651400019 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CELLULAR BIOCHEMISTRY, v.114, no.2, pp.418 - 427 | - |
dc.relation.isPartOf | JOURNAL OF CELLULAR BIOCHEMISTRY | - |
dc.citation.title | JOURNAL OF CELLULAR BIOCHEMISTRY | - |
dc.citation.volume | 114 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 418 | - |
dc.citation.endPage | 427 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | LIFE-SPAN | - |
dc.subject.keywordPlus | INTERFERING RNAS | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | SOMATIC-CELLS | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | LONGEVITY | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | DISEASES | - |
dc.subject.keywordAuthor | DICER-2 | - |
dc.subject.keywordAuthor | DROSOPHILA | - |
dc.subject.keywordAuthor | ENDOGENOUS SIRNA | - |
dc.subject.keywordAuthor | MICROARRAY | - |
dc.subject.keywordAuthor | RNA INTERFERENCE | - |
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