Phorbol 12-Myristate 13-Acetate Enhances Long-Term Potentiation in the Hippocampus through Activation of Protein Kinase C delta and epsilon
DC Field | Value | Language |
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dc.contributor.author | Kim, Eung Chang | - |
dc.contributor.author | Lee, Myeong Jong | - |
dc.contributor.author | Shin, Sang Yep | - |
dc.contributor.author | Seol, Geun Hee | - |
dc.contributor.author | Han, Seung Ho | - |
dc.contributor.author | Yee, Jaeyong | - |
dc.contributor.author | Kim, Chan | - |
dc.contributor.author | Mini, Sun Seek | - |
dc.date.accessioned | 2021-09-06T04:42:42Z | - |
dc.date.available | 2021-09-06T04:42:42Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2013-02 | - |
dc.identifier.issn | 1226-4512 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/104057 | - |
dc.description.abstract | Many intracellular proteins and signaling cascades contribute to the sensitivity of N-methyl-D-aspartate receptors (NMDARs). One such putative contributor is the serine/threonine kinase, protein kinase C (PKC). Activation of PKC by phorbol 12-myristate 13-acetate (PMA) causes activation of extracellular signal-regulated kinase (ERK) and promotes the formation of new spines in cultured hippocampal neurons. The purpose of this study was to examine which PKC isoforms are responsible for the PMA-induced augmentation of long-term potentiation (LTP) in the CA1 stratum radiatum of the hippocampus in vitro and verify that this facilitation requires NMDAR activation. We found that PMA enhanced the induction of LTP by a single episode of theta-burst stimulation in a concentration-dependent manner without affecting to magnitude of baseline field excitatory postsynaptic potentials. Facilitation of LTP by PMA (200 nM) was blocked by the nonspecific PKC inhibitor, Ro 31-8220 (10 mu M); the selective PKC delta inhibitor, rottlerin (1 mu M); and the PKC epsilon inhibitor, TAT- epsilon V1-2 peptide (500 nM). Moreover, the NMDAR blocker DL-APV (50 mu M) prevented enhancement of LTP by PMA. Our results suggest that PMA contributes to synaptic plasticity in the nervous system via activation of PKC delta and/or PKC epsilon, and confirm that NMDAR activity is required for this effect. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY | - |
dc.subject | NMDA RECEPTOR TRAFFICKING | - |
dc.subject | SYNAPTIC PLASTICITY | - |
dc.subject | ER RETENTION | - |
dc.subject | PKM-ZETA | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | MEMORY | - |
dc.subject | CELLS | - |
dc.subject | ALPHA | - |
dc.subject | AUTOPHOSPHORYLATION | - |
dc.subject | INVOLVEMENT | - |
dc.title | Phorbol 12-Myristate 13-Acetate Enhances Long-Term Potentiation in the Hippocampus through Activation of Protein Kinase C delta and epsilon | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Seol, Geun Hee | - |
dc.identifier.doi | 10.4196/kjpp.2013.17.1.51 | - |
dc.identifier.scopusid | 2-s2.0-84876776156 | - |
dc.identifier.wosid | 000334564500008 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, v.17, no.1, pp.51 - 56 | - |
dc.relation.isPartOf | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY | - |
dc.citation.title | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY | - |
dc.citation.volume | 17 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 51 | - |
dc.citation.endPage | 56 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001746110 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Physiology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.subject.keywordPlus | NMDA RECEPTOR TRAFFICKING | - |
dc.subject.keywordPlus | SYNAPTIC PLASTICITY | - |
dc.subject.keywordPlus | ER RETENTION | - |
dc.subject.keywordPlus | PKM-ZETA | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | MEMORY | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | ALPHA | - |
dc.subject.keywordPlus | AUTOPHOSPHORYLATION | - |
dc.subject.keywordPlus | INVOLVEMENT | - |
dc.subject.keywordAuthor | Hippocampus | - |
dc.subject.keywordAuthor | Long-term potentiation (LTP) | - |
dc.subject.keywordAuthor | Phorbol 12-myristate 13-acetate | - |
dc.subject.keywordAuthor | Protein kinase C (PKC) | - |
dc.subject.keywordAuthor | Synaptic plasticity | - |
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