Potentiometric Multichannel Cytometer Microchip for High-throughput Microdispersion Analysis
DC Field | Value | Language |
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dc.contributor.author | Kim, Junhoi | - |
dc.contributor.author | Kim, Eun-Geun | - |
dc.contributor.author | Bae, Sangwook | - |
dc.contributor.author | Kwon, Sunghoon | - |
dc.contributor.author | Chun, Honggu | - |
dc.date.accessioned | 2021-09-06T05:28:59Z | - |
dc.date.available | 2021-09-06T05:28:59Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2013-01-01 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/104233 | - |
dc.description.abstract | The parallelization of microfluidic cytometry is expected to lead to considerably enhanced throughput enabling point-of-care diagnosis. In this article, the development of a microfluidic potentiometric multichannel cytometer is presented. Parallelized microfluidic channels sharing a fluid path inevitably suffer from interchannel signal crosstalk that results from electrical coupling within the microfluidic channel network. By employing three planar electrodes within a single detection channel, we electrically decoupled each channel unit, thereby enabling parallel analysis by using a single cytometer microchip with multiple microfluidic channels. The triple-electrode configuration is validated by analyzing the size and concentration of polystyrene microbeads (diameters: 1.99, 2.58, 3, and 3.68 mu m; concentration range: similar to 2 x 10(5) mL(-1) to similar to 1 x 10(7) mL(-1)) and bacterial rnicrodispersion samples (Bacillus subtilis, concentration range: similar to 4 x 10(5) CFU mL(-1) to similar to 3 x 10(6) CFU mL(-1)). Crosstalk-free parallelized analysis is then demonstrated using a 16-channel potentiometric cytometer (maximum cross-correlation coefficients vertical bar r vertical bar: < 0.13 in all channel combinations). A detection throughput of similar to 48 000 s(-1) was achieved; the throughout can be easily increased with the degree of parallelism of a single microchip without additional technical complexities. Therefore, this methodology should enable high-throughput and low-cost cytometry. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | RESISTIVE PULSE TECHNIQUE | - |
dc.subject | ACTIVATED CELL SORTER | - |
dc.subject | FLOW-CYTOMETRY | - |
dc.subject | COULTER-COUNTER | - |
dc.subject | MICROFLUIDIC CYTOMETER | - |
dc.subject | SUBMICRON PARTICLES | - |
dc.subject | BACILLUS-SUBTILIS | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | SPECTROSCOPY | - |
dc.subject | MICROCHANNELS | - |
dc.title | Potentiometric Multichannel Cytometer Microchip for High-throughput Microdispersion Analysis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chun, Honggu | - |
dc.identifier.doi | 10.1021/ac302905x | - |
dc.identifier.scopusid | 2-s2.0-84871775828 | - |
dc.identifier.wosid | 000313156500054 | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.85, no.1, pp.362 - 368 | - |
dc.relation.isPartOf | ANALYTICAL CHEMISTRY | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 85 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 362 | - |
dc.citation.endPage | 368 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | RESISTIVE PULSE TECHNIQUE | - |
dc.subject.keywordPlus | ACTIVATED CELL SORTER | - |
dc.subject.keywordPlus | FLOW-CYTOMETRY | - |
dc.subject.keywordPlus | COULTER-COUNTER | - |
dc.subject.keywordPlus | MICROFLUIDIC CYTOMETER | - |
dc.subject.keywordPlus | SUBMICRON PARTICLES | - |
dc.subject.keywordPlus | BACILLUS-SUBTILIS | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | SPECTROSCOPY | - |
dc.subject.keywordPlus | MICROCHANNELS | - |
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