Effects of oxygen tension and IGF-I on HIF-1 alpha protein expression in mouse blastocysts
- Authors
- Yoon, Jeong; Juhn, Kyoung-Mi; Ko, Jin-Kyung; Yoon, San-Hyun; Ko, Yong; Lee, Chul-Young; Lim, Jin-Ho
- Issue Date
- 1월-2013
- Publisher
- SPRINGER/PLENUM PUBLISHERS
- Keywords
- HIF-1; IGF-I; Embryo development; Low oxygen tension
- Citation
- JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, v.30, no.1, pp.99 - 105
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
- Volume
- 30
- Number
- 1
- Start Page
- 99
- End Page
- 105
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/104267
- DOI
- 10.1007/s10815-012-9902-z
- ISSN
- 1058-0468
- Abstract
- Hypoxia inducible factors (HIFs) are key regulators of oxygen homeostasis in response to reduced oxygenation in somatic cells. In addition, HIF-1 alpha protein can be also induced by insulin-like growth factor I (IGF-I) treatment in various cell lines under normoxic condition. However, the expression and function of HIF-1 alpha in embryogenesis are still unclear. Therefore, the objectives of this study were to examine the expression of HIF-1 alpha in mouse blastocysts cultured under hypoxic and normoxic conditions, and to determine whether oxygen tension and IGF-I influence embryonic development through stimulation of HIF-1 alpha expression. Mouse embryos were cultured from the 1-cell to blastocyst stage under 5 % or 20 % O-2 in both the absence and presence of IGF-I. The embryonic development rates to the blastocyst stage were not affected by oxygen tension or IGF-I treatment. HIF-1 alpha protein was localized to the cytoplasm of blastocysts, and its levels were independent of oxygen concentration or IGF-I treatment. Blastocysts cultured under 5 % O-2 exhibited significantly higher total cell numbers (83.4 +/- 18.1) and lower apoptotic index (3.7 +/- 1.5) than those cultured under 20 % O-2 (67.4 +/- 15.6) (6.9 +/- 3.5) (P < 0.05). IGF-I reduced the apoptotic index in both oxygen conditions, but a significant decrease was detected in the 20 % O-2 group. HIF-1 alpha may not be a major mediator that responds to change in oxygen tension within blastocysts, inconsistent with that of somatic cells. Supplementation of culture media with IGF-I has been shown to promote embryo development by an anti-apoptotic effect, instead of increasing HIF-1 alpha protein expression.
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