Increased SCF/c-kit by hypoxia promotes autophagy of human placental chorionic plate-derived mesenchymal stem cells via regulating the phosphorylation of mTOR
DC Field | Value | Language |
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dc.contributor.author | Lee, Youjin | - |
dc.contributor.author | Jung, Jieun | - |
dc.contributor.author | Cho, Kyung Jin | - |
dc.contributor.author | Lee, Seoung-Kwan | - |
dc.contributor.author | Park, Jong-Wan | - |
dc.contributor.author | Oh, Il-Hoan | - |
dc.contributor.author | Kim, Gi Jin | - |
dc.date.accessioned | 2021-09-06T05:36:44Z | - |
dc.date.available | 2021-09-06T05:36:44Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2013-01 | - |
dc.identifier.issn | 0730-2312 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/104282 | - |
dc.description.abstract | Hypoxia triggers physiological and pathological cellular processes, including proliferation, differentiation, and death, in several cell types. Mesenchymal stem cells (MSCs) derived from various tissues have self-renewal activity and can differentiate towards multiple lineages. Recently, it has been reported that hypoxic conditions tip the balance between survival and death by hypoxia-induced autophagy, although the underlying mechanism is not clear. The objectives of this study are to compare the effect of hypoxia on the self-renewal of bone marrow-derived mesenchymal stem cells (BM-MSCs) and placental chorionic plate-derived mesenchymal stem cells (CP-MSCs) and to investigate the regulatory mechanisms of self-renewal in each MSC type during hypoxia. The expression of self-renewal markers (e.g., Oct4, Nanog, Sox2) was assessed in both cell lines. PI3K and stem cell factor (SCF) expression gradually increased in CP-MSCs but were markedly downregulated in BM-MSCs by hypoxia. The phosphorylation of ERK and mTOR was augmented by hypoxia in CP-MSCs compared to control. Also, the expression of LC3 II, a component of the autophagosome and the hoof-shaped autophagosome was detected more rapidly in CP-MSCs than in BM-MSCs under hypoxia. Hypoxia induced the expression of SCF in CP-MSCs and increased SCF/c-kit pathway promotes the self-renewal activities of CP-MSCs via an autocrine/paracrine mechanism that balances cell survival and cell death events by autophagy. These activities occur to a greater extent in CP-MSCs than in BM-MSCs through regulating the phosphorylation of mTOR. These findings will provide useful guidelines for better understanding the function of SCF/c-kit in the self-renewal and autophagy-regulated mechanisms that promote of MSC survival. J. Cell. Biochem. 114: 7988, 2012. (C) 2012 Wiley Periodicals, Inc. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | HUMAN TERM PLACENTA | - |
dc.subject | STROMAL CELLS | - |
dc.subject | IN-VITRO | - |
dc.subject | C-KIT | - |
dc.subject | MULTIPOTENT CELLS | - |
dc.subject | DUAL ROLES | - |
dc.subject | CANCER | - |
dc.subject | OXYGEN | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | PROLIFERATION | - |
dc.title | Increased SCF/c-kit by hypoxia promotes autophagy of human placental chorionic plate-derived mesenchymal stem cells via regulating the phosphorylation of mTOR | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Cho, Kyung Jin | - |
dc.contributor.affiliatedAuthor | Lee, Seoung-Kwan | - |
dc.identifier.doi | 10.1002/jcb.24303 | - |
dc.identifier.scopusid | 2-s2.0-84869433016 | - |
dc.identifier.wosid | 000311112700009 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CELLULAR BIOCHEMISTRY, v.114, no.1, pp.79 - 88 | - |
dc.relation.isPartOf | JOURNAL OF CELLULAR BIOCHEMISTRY | - |
dc.citation.title | JOURNAL OF CELLULAR BIOCHEMISTRY | - |
dc.citation.volume | 114 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 79 | - |
dc.citation.endPage | 88 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | HUMAN TERM PLACENTA | - |
dc.subject.keywordPlus | STROMAL CELLS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | C-KIT | - |
dc.subject.keywordPlus | MULTIPOTENT CELLS | - |
dc.subject.keywordPlus | DUAL ROLES | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | OXYGEN | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordAuthor | HYPOXIA | - |
dc.subject.keywordAuthor | BONE MARROW-DERIVED MESENCHYMAL STEM CELLS (BM-MSCs) | - |
dc.subject.keywordAuthor | PLACENTAL CHORIONIC PLATE-DERIVED MESENCHYMAL STEM CELLS (CP-MSCs) | - |
dc.subject.keywordAuthor | STEM CELL FACTOR (SCF) | - |
dc.subject.keywordAuthor | SELF-RENEWAL | - |
dc.subject.keywordAuthor | AUTOPHAGY | - |
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