Aestuariibaculum suncheonense gen. nov., sp nov., a marine bacterium of the family Flavobacteriaceae isolated from a tidal flat and emended descriptions of the genera Gaetbulibacter and Tamlana

Abstract

A Gram-staining-negative, yellow-pigmented, strictly aerobic bacterium, designated strain SC17(T), was isolated from sediment of a tidal flat of Suncheon bay in South Korea. Cells were halotolerant, catalase- and oxidase-positive and non-motile rods. Growth of strain SC17(T) was observed at 5-40 degrees C (optimum, 25-30 degrees C), at pH 6.0-8.5 (optimum, pH 7.0) and in the presence of 1-8% (w/v) NaCl (optimum, 1-2%). The major cellular fatty acids were iso-C-15:0, summed feature 3 (comprising C-16:1 omega 7c and/or C-16:1 omega 6c and/or iso-C-15:0 2-OH), iso-C-17:0 3-OH, iso-C-15:1 G and anteiso-C-15:0. The polar lipid content consisted of phosphatidylethanolamine and unidentified amino lipids and lipids. The G + C content of the genomic DNA was 46.4 mol% and the only respiratory quinone detected was menaquinone-6 (MK-6). Phylogenetic inference based on 16S rRNA gene sequences showed that strain SC17(T) formed a distinct phyletic lineage within the family Flavobacteriaceae and was most closely related to members of the genera Gaetbulibacter and Tamlana with 95.0-95.8% sequence similarity. On the basis of phenotypic and molecular features, strain SC17(T) represents a novel genus of the family Flavobacteriaceae, for which the name Aestuiriibaculum suncheonense gen. nov., sp. nov. is proposed. The type strain is SC17(T) (=KACC 16186(T)=JCM 17789(T)). Emended descriptions of the genera Gaetbulibacter and Tamlana are also proposed.