The Pepper Extracellular Xyloglucan-Specific Endo-beta-1,4-Glucanase Inhibitor Protein Gene, CaXEGIP1, Is Required for Plant Cell Death and Defense Responses
DC Field | Value | Language |
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dc.contributor.author | Choi, Hyong Woo | - |
dc.contributor.author | Kim, Nak Hyun | - |
dc.contributor.author | Lee, Yeon Kyeong | - |
dc.contributor.author | Hwang, Byung Kook | - |
dc.date.accessioned | 2021-09-06T05:54:07Z | - |
dc.date.available | 2021-09-06T05:54:07Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2013-01 | - |
dc.identifier.issn | 0032-0889 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/104385 | - |
dc.description.abstract | Plants produce various proteinaceous inhibitors to protect themselves against microbial pathogen attack. A xyloglucan-specific endo-beta-1,4-glucanase inhibitor1 gene, CaXEGIP1, was isolated and functionally characterized in pepper (Capsicum annuum) plants. CaXEGIP1 was rapidly and strongly induced in pepper leaves infected with avirulent Xanthomonas campestris pv vesicatoria, and purified CaXEGIP1 protein significantly inhibited the hydrolytic activity of the glycoside hydrolase74 family xyloglucan-specific endo-beta-1,4-glucanase from Clostridium thermocellum. Soluble-modified green fluorescent protein-tagged CaXEGIP1 proteins were mainly localized to the apoplast of onion (Allium cepa) epidermal cells. Agrobacterium tumefaciens-mediated overexpression of CaXEGIP1 triggered pathogen-independent, spontaneous cell death in pepper and Nicotiana benthamiana leaves. CaXEGIP1 silencing in pepper conferred enhanced susceptibility to virulent and avirulent X. campestris pv vesicatoria, accompanied by a compromised hypersensitive response and lowered expression of defense-related genes. Overexpression of dexamethasone: CaXEGIP1 in Arabidopsis (Arabidopsis thaliana) enhanced resistance to Hyaloperonospora arabidopsidis infection. Comparative histochemical and proteomic analyses revealed that CaXEGIP1 overexpression induced a spontaneous cell death response and also increased the expression of some defense-related proteins in transgenic Arabidopsis leaves. This response was also accompanied by cell wall thickening and darkening. Together, these results suggest that pathogen-inducible CaXEGIP1 positively regulates cell death-mediated defense responses in plants. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC PLANT BIOLOGISTS | - |
dc.subject | BOTRYTIS-CINEREA | - |
dc.subject | STRUCTURAL BASIS | - |
dc.subject | OXIDATIVE BURST | - |
dc.subject | TOBACCO PLANTS | - |
dc.subject | PATHOGEN | - |
dc.subject | RESISTANCE | - |
dc.subject | EXPRESSION | - |
dc.subject | WALLS | - |
dc.subject | DISEASE | - |
dc.subject | POLYGALACTURONASES | - |
dc.title | The Pepper Extracellular Xyloglucan-Specific Endo-beta-1,4-Glucanase Inhibitor Protein Gene, CaXEGIP1, Is Required for Plant Cell Death and Defense Responses | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Nak Hyun | - |
dc.contributor.affiliatedAuthor | Hwang, Byung Kook | - |
dc.identifier.doi | 10.1104/pp.112.203828 | - |
dc.identifier.scopusid | 2-s2.0-84871817952 | - |
dc.identifier.wosid | 000312964000031 | - |
dc.identifier.bibliographicCitation | PLANT PHYSIOLOGY, v.161, no.1, pp.384 - 396 | - |
dc.relation.isPartOf | PLANT PHYSIOLOGY | - |
dc.citation.title | PLANT PHYSIOLOGY | - |
dc.citation.volume | 161 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 384 | - |
dc.citation.endPage | 396 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.subject.keywordPlus | BOTRYTIS-CINEREA | - |
dc.subject.keywordPlus | STRUCTURAL BASIS | - |
dc.subject.keywordPlus | OXIDATIVE BURST | - |
dc.subject.keywordPlus | TOBACCO PLANTS | - |
dc.subject.keywordPlus | PATHOGEN | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | WALLS | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | POLYGALACTURONASES | - |
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