CD44-negative cells in head and neck squamous carcinoma also have stem-cell like traits
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, Se-Yeong | - |
dc.contributor.author | Kang, Hyun Jung | - |
dc.contributor.author | Kim, Young Sook | - |
dc.contributor.author | Kim, Hyunggee | - |
dc.contributor.author | Lim, Young Chang | - |
dc.date.accessioned | 2021-09-06T05:59:02Z | - |
dc.date.available | 2021-09-06T05:59:02Z | - |
dc.date.created | 2021-06-14 | - |
dc.date.issued | 2013-01 | - |
dc.identifier.issn | 0959-8049 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/104415 | - |
dc.description.abstract | CD44 is generally accepted as a surrogate marker for head and neck squamous carcinoma cancer stem cells (HNSC CSCs) and only CD44+ HNSC cells have tumour initiating capacity. However, a recent report suggested that CSCs themselves might be heterogenous due to various genetic alterations. Here, we compared in vitro stem-like cell characteristics, chemoresistance and in vivo tumour formation capacity of CD44+ and CD44- HNSC cells obtained from primary HNSC patient specimens. CD44- HNSC spheroid cells generated spheroid cells again after seeding of single-dissociated spheroid CD44- HNSC cells. Immunocytochemistry assays revealed that various stem cell markers, including octamer-binding transcription factor 4 (OCT4), sex determining region Y-box 2 (SOX2) and nestin were up-regulated in CD44- spheroid cells, similar to CD44+ spheroid cells. Furthermore, CD44- spheroid cells appeared to be chemoresistant to cisplatin and showed increased levels of ABCG2, similar to CD44+ spheroid cells. Of most interest, as few as 1000 CD44- spheroid cells were able to give rise to tumours in nude mice. The collective data indicate that the cell surface marker CD44 cannot be used as a selective marker of spheroid-forming, tumour-initiating or chemoresistant cell populations, and further indicate the limitation of current HNSC CSC identification methods using the CD44 cell surface marker. (C) 2012 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | HUMAN-MELANOMA CELLS | - |
dc.subject | PROSPECTIVE IDENTIFICATION | - |
dc.subject | INITIATING CELLS | - |
dc.subject | SIDE-POPULATION | - |
dc.subject | CANCER | - |
dc.subject | STATES | - |
dc.subject | TUMORS | - |
dc.subject | CD44 | - |
dc.title | CD44-negative cells in head and neck squamous carcinoma also have stem-cell like traits | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Hyunggee | - |
dc.identifier.doi | 10.1016/j.ejca.2012.06.004 | - |
dc.identifier.scopusid | 2-s2.0-84871417680 | - |
dc.identifier.wosid | 000312896700031 | - |
dc.identifier.bibliographicCitation | EUROPEAN JOURNAL OF CANCER, v.49, no.1, pp.272 - 280 | - |
dc.relation.isPartOf | EUROPEAN JOURNAL OF CANCER | - |
dc.citation.title | EUROPEAN JOURNAL OF CANCER | - |
dc.citation.volume | 49 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 272 | - |
dc.citation.endPage | 280 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | HUMAN-MELANOMA CELLS | - |
dc.subject.keywordPlus | PROSPECTIVE IDENTIFICATION | - |
dc.subject.keywordPlus | INITIATING CELLS | - |
dc.subject.keywordPlus | SIDE-POPULATION | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | STATES | - |
dc.subject.keywordPlus | TUMORS | - |
dc.subject.keywordPlus | CD44 | - |
dc.subject.keywordAuthor | Neoplastic stem cell | - |
dc.subject.keywordAuthor | Head and neck neoplasm | - |
dc.subject.keywordAuthor | Biomarkers | - |
dc.subject.keywordAuthor | CD44 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.